Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 350588..351234 Replicon chromosome
Accession NZ_CP092589
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MKT41_RS01615 Protein ID WP_002920560.1
Coordinates 350588..350935 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MKT41_RS01620 Protein ID WP_002920557.1
Coordinates 350935..351234 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS01605 (MKT41_01605) 346514..347947 + 1434 WP_002920564.1 glycogen synthase GlgA -
MKT41_RS01610 (MKT41_01610) 347965..350412 + 2448 WP_002920561.1 glycogen phosphorylase -
MKT41_RS01615 (MKT41_01615) 350588..350935 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKT41_RS01620 (MKT41_01620) 350935..351234 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MKT41_RS01625 (MKT41_01625) 351297..352805 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MKT41_RS01630 (MKT41_01630) 353010..353339 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MKT41_RS01635 (MKT41_01635) 353390..354220 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MKT41_RS01640 (MKT41_01640) 354270..355028 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
MKT41_RS01645 (MKT41_01645) 355044..355310 - 267 Protein_325 LuxR C-terminal-related transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 355499..356761 1262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T236711 WP_002920560.1 NZ_CP092589:350588-350935 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T236711 NZ_LR699570:c2222445-2222101 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT236711 WP_002920557.1 NZ_CP092589:350935-351234 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT236711 NZ_LR699570:c2222687-2222439 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References