Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2442332..2442849 Replicon chromosome
Accession NZ_CP092554
Organism Staphylococcus aureus strain VMRSA-WC071

Toxin (Protein)


Gene name yoeB Uniprot ID A0A3A3ATA4
Locus tag MKI61_RS12225 Protein ID WP_000113265.1
Coordinates 2442332..2442598 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID T1YB60
Locus tag MKI61_RS12230 Protein ID WP_000584499.1
Coordinates 2442598..2442849 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI61_RS12195 (MKI61_002417) 2437760..2438296 - 537 WP_000169317.1 GNAT family N-acetyltransferase -
MKI61_RS12200 (MKI61_002418) 2438530..2439354 - 825 WP_000572038.1 formate/nitrite transporter family protein -
MKI61_RS12205 (MKI61_002419) 2439571..2439753 - 183 WP_000230292.1 hypothetical protein -
MKI61_RS12210 (MKI61_002420) 2439837..2440304 - 468 WP_000153347.1 SRPBCC domain-containing protein -
MKI61_RS12215 (MKI61_002421) 2440490..2442037 - 1548 WP_000727772.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
MKI61_RS12220 (MKI61_002422) 2442226..2442297 - 72 Protein_2365 hypothetical protein -
MKI61_RS12225 (MKI61_002423) 2442332..2442598 - 267 WP_000113265.1 Txe/YoeB family addiction module toxin Toxin
MKI61_RS12230 (MKI61_002424) 2442598..2442849 - 252 WP_000584499.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MKI61_RS12235 (MKI61_002425) 2443014..2443094 - 81 WP_223197974.1 hypothetical protein -
MKI61_RS12240 (MKI61_002426) 2443122..2443721 - 600 WP_000162810.1 DsbA family protein -
MKI61_RS12245 (MKI61_002427) 2443740..2444102 - 363 WP_000819843.1 DUF4467 domain-containing protein -
MKI61_RS12250 (MKI61_002428) 2444357..2445607 + 1251 WP_001012225.1 FemA/FemB family glycyltransferase FmhA -
MKI61_RS12255 (MKI61_002429) 2445704..2446435 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
MKI61_RS12260 (MKI61_002430) 2446432..2447151 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-81)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10436.96 Da        Isoelectric Point: 10.0573

>T236563 WP_000113265.1 NZ_CP092554:c2442598-2442332 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T236563 NZ_LR698844:2720834-2720974 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT236563 WP_000584499.1 NZ_CP092554:c2442849-2442598 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT236563 NZ_LR698844:c2721108-2720907 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BWF

Antitoxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7BWF
PDB 6L8E
AlphaFold DB T1YB60

References