Detailed information of TA system TA023656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1559944..1560456
• Replicon: chromosome
• Accession: NC_012466
• Organism: Streptococcus pneumoniae JJA

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: C1CFW2
• Locus tag: SPJ_RS08130
• Protein ID: WP_000924718.1
• Coordinates: 1559944..1560198 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: K0ZY22
• Locus tag: SPJ_RS08135
• Protein ID: WP_000388572.1
• Coordinates: 1560202..1560456 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPJ_RS08115	1557045..1557359	-	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
SPJ_RS08120	1557384..1558010	-	627	WP_000775037.1	guanylate kinase	-
SPJ_RS08125	1558140..1559753	-	1614	WP_000404940.1	ribonuclease Y	-
SPJ_RS08130	1559944..1560198	-	255	WP_000924718.1	Txe/YoeB family addiction module toxin	Toxin
SPJ_RS08135	1560202..1560456	-	255	WP_000388572.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SPJ_RS08140	1560694..1561791	-	1098	WP_000156351.1	nucleotidyltransferase	-
SPJ_RS08145	1561801..1562547	-	747	WP_000605161.1	class I SAM-dependent methyltransferase	-
SPJ_RS08150	1562650..1563003	-	354	WP_001003001.1	ribosome silencing factor	-
SPJ_RS08155	1563016..1563516	-	501	WP_000781356.1	cysteine hydrolase	-
SPJ_RS08160	1563517..1564110	-	594	WP_000121674.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SPJ_RS08165	1564110..1564739	-	630	WP_000151309.1	nicotinate-nucleotide adenylyltransferase	-
SPJ_RS08170	1564791..1565102	-	312	WP_000060169.1	ribosome assembly RNA-binding protein YhbY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10131.55 Da
• Isoelectric Point: 7.9716

>T23656 WP_000924718.1 NC_012466:c1560198-1559944 [Streptococcus pneumoniae JJA]
MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIAKPEPLKYDYQGAWSRRIDAENRLIYMTDGDSMAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T23656 NC_012466:c1560198-1559944 [Streptococcus pneumoniae JJA]
ATGCTACTCAAGTTTACAGAAGATGCGTGGGCAGATTATTGCTACTGGCAAAACCAAGATAAAAAAACACTAAAAAGAAT
CAATAAATTAATCAAGGATATTCAACGCGATCCATTTACAGGAATAGCAAAACCAGAGCCACTCAAATATGATTACCAAG
GAGCCTGGTCACGGCGTATTGATGCAGAAAATCGCTTGATTTATATGACGGATGGAGATAGCATGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9706.00 Da
• Isoelectric Point: 4.8430

>AT23656 WP_000388572.1 NC_012466:c1560456-1560202 [Streptococcus pneumoniae JJA]
MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKSEWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVH
VIEE

Nucleotide

• Length: 255 bp

>AT23656 NC_012466:c1560456-1560202 [Streptococcus pneumoniae JJA]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGATGAGGACATTGTAGTTCTTTCAAAGAGCGAATGGGACAGTATTCAGGAAACCCTGA
GAATCGCCCAAAATAAGGAGTTATCAGACAAGGTTTTGCGAGGAATGGCTCAAGTCCGTGCTGGAAGCACTCAGGTTCAT
GTGATTGAGGAGTGA

Structures

Toxin

• AlphaFold DB: C1CFW2

Antitoxin

• AlphaFold DB: K0ZY22