Detailed information of TA system TA236539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2442627..2443144
• Replicon: chromosome
• Accession: NZ_CP092550
• Organism: Staphylococcus aureus strain VMRSA-WC082

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: MKI63_RS12230
• Protein ID: WP_000113265.1
• Coordinates: 2442627..2442893 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: MKI63_RS12235
• Protein ID: WP_000584499.1
• Coordinates: 2442893..2443144 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI63_RS12200 (MKI63_002414)	2438055..2438591	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
MKI63_RS12205 (MKI63_002415)	2438825..2439649	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
MKI63_RS12210 (MKI63_002416)	2439866..2440048	-	183	WP_000230292.1	hypothetical protein	-
MKI63_RS12215 (MKI63_002417)	2440132..2440599	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
MKI63_RS12220 (MKI63_002418)	2440785..2442332	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
MKI63_RS12225 (MKI63_002419)	2442521..2442592	-	72	Protein_2367	hypothetical protein	-
MKI63_RS12230 (MKI63_002420)	2442627..2442893	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
MKI63_RS12235 (MKI63_002421)	2442893..2443144	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI63_RS12240 (MKI63_002422)	2443309..2443389	-	81	WP_223197974.1	hypothetical protein	-
MKI63_RS12245 (MKI63_002423)	2443417..2444016	-	600	WP_000162810.1	DsbA family protein	-
MKI63_RS12250 (MKI63_002424)	2444035..2444397	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
MKI63_RS12255 (MKI63_002425)	2444652..2445902	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
MKI63_RS12260 (MKI63_002426)	2445999..2446730	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
MKI63_RS12265 (MKI63_002427)	2446727..2447446	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T236539 WP_000113265.1 NZ_CP092550:c2442893-2442627 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T236539 NZ_LR595899:535107-535376 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT236539 WP_000584499.1 NZ_CP092550:c2443144-2442893 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT236539 NZ_LR595899:535360-535644 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60