Detailed information of TA system TA236503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2415921..2416438
• Replicon: chromosome
• Accession: NZ_CP092542
• Organism: Staphylococcus aureus strain VRMSSA-WC113

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: MKI55_RS12065
• Protein ID: WP_000113265.1
• Coordinates: 2415921..2416187 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: MKI55_RS12070
• Protein ID: WP_000584499.1
• Coordinates: 2416187..2416438 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI55_RS12035 (MKI55_002389)	2411349..2411885	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
MKI55_RS12040 (MKI55_002390)	2412119..2412943	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
MKI55_RS12045 (MKI55_002391)	2413160..2413342	-	183	WP_000230292.1	hypothetical protein	-
MKI55_RS12050 (MKI55_002392)	2413426..2413893	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
MKI55_RS12055 (MKI55_002393)	2414079..2415626	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
MKI55_RS12060 (MKI55_002394)	2415815..2415886	-	72	Protein_2330	hypothetical protein	-
MKI55_RS12065 (MKI55_002395)	2415921..2416187	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
MKI55_RS12070 (MKI55_002396)	2416187..2416438	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI55_RS12075 (MKI55_002397)	2416603..2416683	-	81	WP_223197974.1	hypothetical protein	-
MKI55_RS12080 (MKI55_002398)	2416711..2417310	-	600	WP_000162810.1	DsbA family protein	-
MKI55_RS12085 (MKI55_002399)	2417329..2417691	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
MKI55_RS12090 (MKI55_002400)	2417946..2419196	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
MKI55_RS12095 (MKI55_002401)	2419293..2420024	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
MKI55_RS12100 (MKI55_002402)	2420021..2420740	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T236503 WP_000113265.1 NZ_CP092542:c2416187-2415921 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T236503 NZ_LR595691:c2120635-2120381 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT236503 WP_000584499.1 NZ_CP092542:c2416438-2416187 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT236503 NZ_LR595691:c2120883-2120632 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60