Detailed information of TA system TA236491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2442606..2443123
• Replicon: chromosome
• Accession: NZ_CP092540
• Organism: Staphylococcus aureus strain VMRSA-WC121

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: MKI67_RS12215
• Protein ID: WP_000113265.1
• Coordinates: 2442606..2442872 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: MKI67_RS12220
• Protein ID: WP_000584499.1
• Coordinates: 2442872..2443123 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI67_RS12185 (MKI67_002418)	2438034..2438570	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
MKI67_RS12190 (MKI67_002419)	2438804..2439628	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
MKI67_RS12195 (MKI67_002420)	2439845..2440027	-	183	WP_000230292.1	hypothetical protein	-
MKI67_RS12200 (MKI67_002421)	2440111..2440578	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
MKI67_RS12205 (MKI67_002422)	2440764..2442311	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
MKI67_RS12210 (MKI67_002423)	2442500..2442571	-	72	Protein_2360	hypothetical protein	-
MKI67_RS12215 (MKI67_002424)	2442606..2442872	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
MKI67_RS12220 (MKI67_002425)	2442872..2443123	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI67_RS12225 (MKI67_002426)	2443288..2443368	-	81	WP_223197974.1	hypothetical protein	-
MKI67_RS12230 (MKI67_002427)	2443396..2443995	-	600	WP_000162810.1	DsbA family protein	-
MKI67_RS12235 (MKI67_002428)	2444014..2444376	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
MKI67_RS12240 (MKI67_002429)	2444631..2445881	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
MKI67_RS12245 (MKI67_002430)	2445978..2446709	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
MKI67_RS12250 (MKI67_002431)	2446706..2447425	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T236491 WP_000113265.1 NZ_CP092540:c2442872-2442606 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T236491 NZ_LR594690:c548603-548322 [Variovorax sp. WDL1]
ATGCGGCAGATTGAGCGCACCGGCCAGTTCAAGCGCGACTACAAGCGCGAGGCGAAAGGGCAGCACCGGGCCACGCTCGA
CGCCGACCTGGTGCCCGTCCTGGTCGCGCTCGCCGACGACCAGCCGCTGGAACCTCGGCACCGCGACCACGCGCTGACCG
GGGACTGGAAGGATCACCGGGATTGCCACGTCAAGCCCGACCTGGTGCTGATCTACCAGAAGCCCGACGCCGACACGCTG
CGCCTGGTGCGCCTCGGCTCTCACTCCGAACTCGGCTTGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT236491 WP_000584499.1 NZ_CP092540:c2443123-2442872 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT236491 NZ_LR594690:c548853-548590 [Variovorax sp. WDL1]
ATGGCTGCAAACCAGCTCGTACAAACGCGCATCGACGGCGCGATCAAGGAAGAAGCGGCGGCCGTCCTGGCCGCGATGGG
CCTTACCGTGTCCGACGCGGTGCGGCTGCTGCTCACGAAGGTGGCCCAGGACAAGGCGCTGCCCTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCCCGCAAGGGCAAGCTGCCGCGCTTCGCCACCGTCAACGATCTGATG
GCCGATCTGCATGCGGCAGATTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60