Detailed information of TA system TA236445

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-timR/SymE(toxin)
• Location: 4110704..4111116
• Replicon: chromosome
• Accession: NZ_CP092534
• Organism: Escherichia coli strain DS566

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: MKD44_RS19990
• Protein ID: WP_000132614.1
• Coordinates: 4110775..4111116 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	timR
Locus tag	-
Coordinates	4110704..4110780 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD44_RS19980 (4107316)	4107316..4108785	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
MKD44_RS19985 (4108785)	4108785..4110554	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (4110704)	4110704..4110780	-	77	NuclAT_8	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_8	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_8	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_8	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_9	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_9	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_9	-	Antitoxin
- (4110704)	4110704..4110780	-	77	NuclAT_9	-	Antitoxin
MKD44_RS19990 (4110775)	4110775..4111116	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
MKD44_RS19995 (4111163)	4111163..4112326	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
MKD44_RS20000 (4112380)	4112380..4113256	-	877	Protein_3913	DUF262 domain-containing protein	-
MKD44_RS20005 (4113662)	4113662..4114582	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
MKD44_RS20010 (4114767)	4114767..4116047	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4095092..4111116	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T236445 WP_000132614.1 NZ_CP092534:4110775-4111116 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T236445 NZ_LR594047:c948017-947172 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT236445 NZ_CP092534:c4110780-4110704 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin