Detailed information of TA system TA236441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3719584..3720278
• Replicon: chromosome
• Accession: NZ_CP092534
• Organism: Escherichia coli strain DS566

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MKD44_RS18115
• Protein ID: WP_001263500.1
• Coordinates: 3719584..3719982 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MKD44_RS18120
• Protein ID: WP_000554758.1
• Coordinates: 3719985..3720278 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD44_RS18090 (3714949)	3714949..3715407	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
MKD44_RS18095 (3715668)	3715668..3717125	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MKD44_RS18100 (3717182)	3717182..3717796	-	615	WP_000602124.1	peptide chain release factor H	-
MKD44_RS18105 (3717793)	3717793..3718932	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
MKD44_RS18110 (3719122)	3719122..3719574	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MKD44_RS18115 (3719584)	3719584..3719982	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MKD44_RS18120 (3719985)	3719985..3720278	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MKD44_RS18125 (3720330)	3720330..3721385	-	1056	WP_001226168.1	DNA polymerase IV	-
MKD44_RS18130 (3721456)	3721456..3722379	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
MKD44_RS18135 (3722382)	3722382..3723245	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
MKD44_RS18140 (3723258)	3723258..3723974	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
MKD44_RS18145 (3723994)	3723994..3724461	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T236441 WP_001263500.1 NZ_CP092534:c3719982-3719584 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T236441 NZ_LR594045:c561412-561071 [Streptococcus dysgalactiae subsp. equisimilis]
ATGGCCTATAATGTACAAATTACCAAAAGTGCCGAGAGAGATCTAGACAGTATCTATCAGTTCTATCTAGATGAATTTTC
TGAACAAAGTGCTTTGAAAGTTATCAAAAGCCTTCAAGATGCTATCAGCTTTCTTTATGTTTCACCACTTGGCTACACCG
ACTTCGATTCTAAAATCAATCGAAAAATTTATCCACAAGGTAATCTAAGAATGATTCCTTCAAAGCAATACCTTATTTTT
TATCTTATCCGTGAAGAAAGGGTAGATGTTTTAAGAATTATTCATTCTAGAACAGATTATCTCAATAATTTAGAAAATCT
CTTTAAAAATCTAAACCTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT236441 WP_000554758.1 NZ_CP092534:c3720278-3719985 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT236441 NZ_LR594045:c561689-561402 [Streptococcus dysgalactiae subsp. equisimilis]
ATGAATACACTAGCAAGAGATCGCCAATATAATTTCCGTGTCAATGCGGATATGCTAAATCAGGCTAAGGAAATTTTGGA
GGAAAAACATATGTCTCTACCTGATGCTCTTAATTTATTTGTAGAGCAAGTGGTTGTCACTAAAAACCTCCCTATCAAAA
CACCTGAGCAAGTTCAAGCTGAATCTTTCTTAAATGAATTAATTTCAGAACTTGATGAAGGCTATCAAGATGTTCTAAAA
GGAAATACAAAACCAGCTAATGAGGTTTTTGCAAAATATGGCCTATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3