Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3491849..3492528 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag MKD44_RS17070 Protein ID WP_000057523.1
Coordinates 3492226..3492528 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MKD44_RS17065 Protein ID WP_000806442.1
Coordinates 3491849..3492190 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS17055 (3488093) 3488093..3489025 - 933 WP_001538399.1 glutaminase A -
MKD44_RS17060 (3489287) 3489287..3491791 + 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
MKD44_RS17065 (3491849) 3491849..3492190 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MKD44_RS17070 (3492226) 3492226..3492528 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKD44_RS17075 (3492661) 3492661..3493455 + 795 WP_001538398.1 TraB/GumN family protein -
MKD44_RS17080 (3493659) 3493659..3494138 + 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MKD44_RS17085 (3494175) 3494175..3495827 - 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
MKD44_RS17090 (3496045) 3496045..3497265 + 1221 WP_001773866.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T236439 WP_000057523.1 NZ_CP092534:c3492528-3492226 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T236439 NZ_LR594043:c1892100-1891843 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAAGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAACGACACCCGTTTGAAGGTATTGCCAAGCCAGAACCTTTGAAGTATGATTATAAGG
GAGCCTGGTCGAGACGGATTGATGCTGAGAATCGGTTGATTTATATGGTAGAAACCGACCAGTTGTATATCTTGTCGCTG
AAGGACCATTATGGGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT236439 WP_000806442.1 NZ_CP092534:c3492190-3491849 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT236439 NZ_LR594043:c1892362-1892102 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTTTTGCGTGGTATGGCTCAAGTCAAAGCTGGACAAACCCAAGAACAC
AATCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References