Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2450603..2451165 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MKD44_RS11785 Protein ID WP_000605675.1
Coordinates 2450603..2450881 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MKD44_RS11790 Protein ID WP_000781370.1
Coordinates 2450881..2451165 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS11760 (2446189) 2446189..2446620 - 432 WP_000152305.1 peroxiredoxin OsmC -
MKD44_RS11765 (2446965) 2446965..2447180 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MKD44_RS11770 (2447282) 2447282..2447419 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MKD44_RS11775 (2447576) 2447576..2449273 + 1698 WP_000433460.1 malate dehydrogenase -
MKD44_RS11780 (2449407) 2449407..2450417 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MKD44_RS11785 (2450603) 2450603..2450881 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKD44_RS11790 (2450881) 2450881..2451165 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MKD44_RS11795 (2451394) 2451394..2452047 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MKD44_RS11800 (2452040) 2452040..2452924 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MKD44_RS11805 (2452937) 2452937..2455984 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T236438 WP_000605675.1 NZ_CP092534:2450603-2450881 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T236438 NZ_LR594043:c1413926-1413666 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT236438 WP_000781370.1 NZ_CP092534:2450881-2451165 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT236438 NZ_LR594043:c1414149-1413928 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References