Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 661689..662416 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MKD44_RS03215 Protein ID WP_000550189.1
Coordinates 661689..662003 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MKD44_RS03220 Protein ID WP_000560266.1
Coordinates 662000..662416 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS03195 (657847) 657847..658833 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
MKD44_RS03200 (658912) 658912..659604 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
MKD44_RS03205 (659681) 659681..660184 - 504 WP_001333820.1 M48 family metallopeptidase -
MKD44_RS03210 (660269) 660269..661405 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MKD44_RS03215 (661689) 661689..662003 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MKD44_RS03220 (662000) 662000..662416 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MKD44_RS03225 (662461) 662461..664479 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MKD44_RS03230 (664905) 664905..667256 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T236425 WP_000550189.1 NZ_CP092534:661689-662003 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T236425 NZ_LR594038:832546-832956 [Klebsiella grimontii]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGTTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT236425 WP_000560266.1 NZ_CP092534:662000-662416 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT236425 NZ_LR594038:832299-832565 [Klebsiella grimontii]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References