Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3719562..3720256 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag MKD47_RS18110 Protein ID WP_001263500.1
Coordinates 3719562..3719960 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MKD47_RS18115 Protein ID WP_000554758.1
Coordinates 3719963..3720256 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS18085 (3714927) 3714927..3715385 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
MKD47_RS18090 (3715646) 3715646..3717103 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
MKD47_RS18095 (3717160) 3717160..3717774 - 615 WP_000602124.1 peptide chain release factor H -
MKD47_RS18100 (3717771) 3717771..3718910 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
MKD47_RS18105 (3719100) 3719100..3719552 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
MKD47_RS18110 (3719562) 3719562..3719960 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MKD47_RS18115 (3719963) 3719963..3720256 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MKD47_RS18120 (3720308) 3720308..3721363 - 1056 WP_001226168.1 DNA polymerase IV -
MKD47_RS18125 (3721434) 3721434..3722357 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
MKD47_RS18130 (3722360) 3722360..3723223 - 864 WP_001169518.1 flagellar motor stator protein MotA -
MKD47_RS18135 (3723236) 3723236..3723952 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
MKD47_RS18140 (3723972) 3723972..3724439 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T236413 WP_001263500.1 NZ_CP092533:c3719960-3719562 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T236413 NZ_LR590625:c825336-824491 [Streptococcus canis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT236413 WP_000554758.1 NZ_CP092533:c3720256-3719963 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT236413 NZ_LR590625:c825923-825333 [Streptococcus canis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References