236410

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2450582..2451144 Replicon chromosome
Accession NZ_CP092533
Organism Escherichia coli strain DS566-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MKD47_RS11780 Protein ID WP_000605675.1
Coordinates 2450582..2450860 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MKD47_RS11785 Protein ID WP_000781370.1
Coordinates 2450860..2451144 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD47_RS11755 (2446168) 2446168..2446599 - 432 WP_000152305.1 peroxiredoxin OsmC -
MKD47_RS11760 (2446944) 2446944..2447159 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MKD47_RS11765 (2447261) 2447261..2447398 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MKD47_RS11770 (2447555) 2447555..2449252 + 1698 WP_000433460.1 malate dehydrogenase -
MKD47_RS11775 (2449386) 2449386..2450396 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MKD47_RS11780 (2450582) 2450582..2450860 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKD47_RS11785 (2450860) 2450860..2451144 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MKD47_RS11790 (2451373) 2451373..2452026 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MKD47_RS11795 (2452019) 2452019..2452903 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MKD47_RS11800 (2452916) 2452916..2455963 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T236410 WP_000605675.1 NZ_CP092533:2450582-2450860 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T236410 NZ_LR590482:5591213-5591494 [Pseudomonas synxantha]
ATGACCCAGCTCAAGCCATTTGAGGCGTCTGAATACCTTACTAACGAGGACACCATCAGTGAATTCCTCACTGCCTCGCT
TGAAGACCCAAACCCTGACGTGTTCCTGCGCGCCTTGCAAGAAGTCGCCAAGGCCCGTGGTATGTCGCAGTTGGCCCGTG
ATGCAGGCATGAACCGTGAGAGCCTGTACAAAGCGTTGGCACCAGGTGCAAAGCCCCGGTATGACACAGTGTTGAAGCTT
GTGAATGCATTAGGCGTGAAGCTGACGGCACAGCACGTCTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT236410 WP_000781370.1 NZ_CP092533:2450860-2451144 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT236410 NZ_LR590482:5590908-5591216 [Pseudomonas synxantha]
ATGAACACACTCATCACAACAACAGAATTCAACCAGTGGTTGAAAGGCTTGAAGGACGACAGGGCACGAGCCCGGATCGT
CTCGCGGATGGTTTCAGCACAGGAAGGTAATTTCGGTGACTGTGAGCCGGTAGGTGATGGGATTTCAGAGATGCGGATCT
TCGTCGGCCCTGGCTACCGCGTCTACTACACGCGCCGTGGAGAAGTTGTGTATCTGTTACTGTGCGGTGGTGACAAGTCG
TCGCAAAAGCGCGACATAAGGGCCGCAAAAGCTATCCTAAACGAGATCGATCGTGGAGAACGTACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References