Detailed information of TA system TA023641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3498215..3498885
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: JTY_RS16485
• Protein ID: WP_003899954.1
• Coordinates: 3498215..3498559 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: JTY_RS16490
• Protein ID: WP_003899955.1
• Coordinates: 3498556..3498885 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS16455	3493288..3494148	+	861	WP_003416628.1	alpha/beta hydrolase	-
JTY_RS16460	3494123..3494638	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
JTY_RS16465	3494878..3495171	+	294	WP_003416635.1	hypothetical protein	-
JTY_RS16470	3495459..3496748	+	1290	WP_003416640.1	ATP-binding protein	-
JTY_RS16475	3497095..3497529	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
JTY_RS16480	3497532..3497984	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JTY_RS16485	3498215..3498559	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JTY_RS16490	3498556..3498885	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
JTY_RS16495	3499423..3499770	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
JTY_RS16500	3499767..3500387	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
JTY_RS16505	3500547..3501812	-	1266	WP_003909837.1	hypothetical protein	-
JTY_RS16510	3501980..3502189	+	210	WP_003416778.1	hypothetical protein	-
JTY_RS16520	3502436..3503470	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T23641 WP_003899954.1 NC_012207:3498215-3498559 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T23641 NC_012207:3498215-3498559 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT23641 WP_003899955.1 NC_012207:3498556-3498885 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT23641 NC_012207:3498556-3498885 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6