Detailed information of TA system TA236389

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-timR/SymE(toxin)
• Location: 4110650..4111062
• Replicon: chromosome
• Accession: NZ_CP092532
• Organism: Escherichia coli strain DS566-2

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: MKD45_RS19990
• Protein ID: WP_000132614.1
• Coordinates: 4110721..4111062 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	timR
Locus tag	-
Coordinates	4110650..4110726 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD45_RS19980 (4107262)	4107262..4108731	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
MKD45_RS19985 (4108731)	4108731..4110500	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (4110650)	4110650..4110726	-	77	NuclAT_8	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_8	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_8	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_8	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_9	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_9	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_9	-	Antitoxin
- (4110650)	4110650..4110726	-	77	NuclAT_9	-	Antitoxin
MKD45_RS19990 (4110721)	4110721..4111062	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
MKD45_RS19995 (4111109)	4111109..4112272	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
MKD45_RS20000 (4112326)	4112326..4113202	-	877	Protein_3913	DUF262 domain-containing protein	-
MKD45_RS20005 (4113608)	4113608..4114528	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
MKD45_RS20010 (4114713)	4114713..4115993	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4095038..4111062	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T236389 WP_000132614.1 NZ_CP092532:4110721-4111062 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T236389 NZ_LR590469:c3246062-3245643 [Yersinia enterocolitica subsp. enterocolitica]
ATGATCAAAAAATTATATATGCTTGATACGAATATTTGTTCTTTTATTATGCGGGAACGACCAGCAGAATTAATTATAAA
ACTACAACAGTGTATTGAGCATCAGAATAAAATAGTTGTCTCAGCAATTACATACTCAGAAATGCGGTTTGGCGCTATAG
GTAAGAAAGCATCTCCAAATCATAATTATTTAGTTGATGAATCTGTAAAAAGGTTAGATGCTATTTTACCGTGGGATACC
GCAGCTGTTAATGCCACGACTAATATTAAGGTTGAGCTGGCAAAGCAGGGGACATCAATTGGTGGAAATGATGCCGCCAT
AGCAGGGCATGCTATTTCTGTTGGCGCAATATTAGTCACTAATAATATTGGAGAATTTGAAAGAGTCAAAAAGTTGAGAA
TAGAAGACCGGACTCTATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT236389 NZ_CP092532:c4110726-4110650 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin