236385

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3719585..3720279 Replicon chromosome
Accession NZ_CP092532
Organism Escherichia coli strain DS566-2

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag MKD45_RS18115 Protein ID WP_001263500.1
Coordinates 3719585..3719983 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MKD45_RS18120 Protein ID WP_000554758.1
Coordinates 3719986..3720279 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD45_RS18090 (3714950) 3714950..3715408 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
MKD45_RS18095 (3715669) 3715669..3717126 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
MKD45_RS18100 (3717183) 3717183..3717797 - 615 WP_000602124.1 peptide chain release factor H -
MKD45_RS18105 (3717794) 3717794..3718933 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
MKD45_RS18110 (3719123) 3719123..3719575 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
MKD45_RS18115 (3719585) 3719585..3719983 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MKD45_RS18120 (3719986) 3719986..3720279 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MKD45_RS18125 (3720331) 3720331..3721386 - 1056 WP_001226168.1 DNA polymerase IV -
MKD45_RS18130 (3721457) 3721457..3722380 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
MKD45_RS18135 (3722383) 3722383..3723246 - 864 WP_001169518.1 flagellar motor stator protein MotA -
MKD45_RS18140 (3723259) 3723259..3723975 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
MKD45_RS18145 (3723995) 3723995..3724462 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T236385 WP_001263500.1 NZ_CP092532:c3719983-3719585 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T236385 NZ_LR590469:c1335677-1335583 [Yersinia enterocolitica subsp. enterocolitica]
AACAAGCCTACGTTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATCGT
ATTCGGTCTTTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT236385 WP_000554758.1 NZ_CP092532:c3720279-3719986 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT236385 NZ_LR590469:1335581-1335699 [Yersinia enterocolitica subsp. enterocolitica]
ATAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAACGTAGGCTTGTTCAGCCATACTCTTTAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References