Detailed information of TA system TA236383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3491847..3492526
• Replicon: chromosome
• Accession: NZ_CP092532
• Organism: Escherichia coli strain DS566-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: MKD45_RS17070
• Protein ID: WP_000057523.1
• Coordinates: 3492224..3492526 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: MKD45_RS17065
• Protein ID: WP_000806442.1
• Coordinates: 3491847..3492188 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD45_RS17055 (3488091)	3488091..3489023	-	933	WP_001538399.1	glutaminase A	-
MKD45_RS17060 (3489285)	3489285..3491789	+	2505	WP_000083948.1	copper-exporting P-type ATPase CopA	-
MKD45_RS17065 (3491847)	3491847..3492188	-	342	WP_000806442.1	HigA family addiction module antitoxin	Antitoxin
MKD45_RS17070 (3492224)	3492224..3492526	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKD45_RS17075 (3492659)	3492659..3493453	+	795	WP_001538398.1	TraB/GumN family protein	-
MKD45_RS17080 (3493657)	3493657..3494136	+	480	WP_001538397.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
MKD45_RS17085 (3494173)	3494173..3495825	-	1653	WP_001773867.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
MKD45_RS17090 (3496043)	3496043..3497263	+	1221	WP_001773866.1	fosmidomycin MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T236383 WP_000057523.1 NZ_CP092532:c3492526-3492224 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T236383 NZ_LR590469:1206406-1206810 [Yersinia enterocolitica subsp. enterocolitica]
ATGCATAAGTACATGCTCGATACGAATATCGTTATATATGTCATTAAACGCCGGCCTATAGAAGTGTTAGGTCGGTTTAA
CGCTAATGCGGGCAGAATGGTTATTTCTTCCATCACACTGGCTGAGTTATTGCATGGCGTAGAGAAAAGCGCGGCACCTG
AACGCAATTTAAGTGTTGTAGAGGATTTTGTTTCACGTCTTACTGTATTGGATTATGACGATAAAGCCGCAATTCACTAT
GGTTCTATACGTGCGAGTCTGGAGCAAAAAGGCACACCCATCGGTGTTAATGATTTGCATATCGCAGGTCATGCCAGAAG
CACCGGATTGATATTAGTGACCAATAATCGCAAAGAATTTGATCGTGTGCCTGGACTGATTGTCGACAACTGGCTAGGAG
AATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT236383 WP_000806442.1 NZ_CP092532:c3492188-3491847 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT236383 NZ_LR590469:1206173-1206406 [Yersinia enterocolitica subsp. enterocolitica]
ATGTCACAGGGCACCATTTTTACGAACAACCGTACTCAAAACGTGCGTTTACCGGTGGATATGCGCTTTCCAGAGAATGT
TAAATCAGTGACTGTGAGAGCTGTAGGGAATGAGCGTATCTTGTCACCCGTTGAAAACTCTTGGGATAGCTTTTTCTTCA
GTAGTCCGCGTGTTTCAGACGATTTTCTGACCGAACGCGCAGAACAGCATCAATCAGAGCGTGAGTTATTTTAA

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3