Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 2450598..2451160 | Replicon | chromosome |
| Accession | NZ_CP092532 | ||
| Organism | Escherichia coli strain DS566-2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | S1Q1V8 |
| Locus tag | MKD45_RS11780 | Protein ID | WP_000605675.1 |
| Coordinates | 2450598..2450876 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | S1PAQ1 |
| Locus tag | MKD45_RS11785 | Protein ID | WP_000781370.1 |
| Coordinates | 2450876..2451160 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKD45_RS11755 (2446184) | 2446184..2446615 | - | 432 | WP_000152305.1 | peroxiredoxin OsmC | - |
| MKD45_RS11760 (2446960) | 2446960..2447175 | + | 216 | WP_000495766.1 | biofilm-dependent modulation protein | - |
| MKD45_RS11765 (2447277) | 2447277..2447414 | + | 138 | WP_000841554.1 | stationary-phase-induced ribosome-associated protein | - |
| MKD45_RS11770 (2447571) | 2447571..2449268 | + | 1698 | WP_000433460.1 | malate dehydrogenase | - |
| MKD45_RS11775 (2449402) | 2449402..2450412 | + | 1011 | WP_000642412.1 | alcohol dehydrogenase AdhP | - |
| MKD45_RS11780 (2450598) | 2450598..2450876 | + | 279 | WP_000605675.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| MKD45_RS11785 (2450876) | 2450876..2451160 | + | 285 | WP_000781370.1 | HigA family addiction module antitoxin | Antitoxin |
| MKD45_RS11790 (2451389) | 2451389..2452042 | - | 654 | WP_000045647.1 | formate dehydrogenase-N subunit gamma | - |
| MKD45_RS11795 (2452035) | 2452035..2452919 | - | 885 | WP_001240584.1 | formate dehydrogenase N subunit beta | - |
| MKD45_RS11800 (2452932) | 2452932..2455979 | - | 3048 | WP_011478186.1 | formate dehydrogenase-N subunit alpha | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10537.16 Da Isoelectric Point: 7.3206
>T236382 WP_000605675.1 NZ_CP092532:2450598-2450876 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH
Download Length: 279 bp
>T236382 NZ_LR590469:c992983-992525 [Yersinia enterocolitica subsp. enterocolitica]
ATGATGCTCTACCGCATTGTGATGCGCCGCTATCTTGCCTCAACCTGGACCGGCTATGGTGCCGAAACCTATGGTGGGCG
CTGGAACCATAAGGGGCACGCCGCCATCTACTTAGCCAGCAGTGTATCACTGGCGATGTTGGAGACGTTGGTACATATTC
AGGACAGCTCAACACTGAGTGAGTTCGAGTTATTCCAAATTGAAATCGAAGATAGCAACATTATGCTGCTTCAGCCGCAA
GATTGGCCCACGAACTGGCGCAGCGACCCCGCACCTGCGACCACAATGGATATCGGCACTGAATGGCTGGAGTCAGAATC
ATCACTTGGATTACTGGTTCCATCCACTCTGGTGCCGACAGAAAACAATCTACTGCTCAATCCTCGGCACAAAAGCTTTC
AAACCTGCTTAAGCAGTGTTCAACCACTCTCTTTTGCCTTTGACCCACGGCTGAAATAG
ATGATGCTCTACCGCATTGTGATGCGCCGCTATCTTGCCTCAACCTGGACCGGCTATGGTGCCGAAACCTATGGTGGGCG
CTGGAACCATAAGGGGCACGCCGCCATCTACTTAGCCAGCAGTGTATCACTGGCGATGTTGGAGACGTTGGTACATATTC
AGGACAGCTCAACACTGAGTGAGTTCGAGTTATTCCAAATTGAAATCGAAGATAGCAACATTATGCTGCTTCAGCCGCAA
GATTGGCCCACGAACTGGCGCAGCGACCCCGCACCTGCGACCACAATGGATATCGGCACTGAATGGCTGGAGTCAGAATC
ATCACTTGGATTACTGGTTCCATCCACTCTGGTGCCGACAGAAAACAATCTACTGCTCAATCCTCGGCACAAAAGCTTTC
AAACCTGCTTAAGCAGTGTTCAACCACTCTCTTTTGCCTTTGACCCACGGCTGAAATAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10401.08 Da Isoelectric Point: 8.8567
>AT236382 WP_000781370.1 NZ_CP092532:2450876-2451160 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ
Download Length: 285 bp
>AT236382 NZ_LR590469:c993485-993039 [Yersinia enterocolitica subsp. enterocolitica]
ATGAGAGCGTATCACCCCACTCCAGCGACCAAAACAAGGGCGCTATGGCGAGAGATTGGTTTGCCCGCCAGCCGCGGTAC
GGTATTGGTCGATAGCATCAAAATGGGTTTTTCTGTTGATGTCATTGACAGCATCCATCTATGGGCATCTATCCCAAAAG
CTGAAATCTTGCGTGCGACCGGTATTCCCAGCCGCAGCTTGACTCGCAGACGCACCCATGATGGCCGTTTCACTCCCGAA
GAGAGTGAGCGCATTGCGCGTTTCGTTCGGGTTATGGACGCCGCAGTTGATCTATTTGGCGGAGATAAAGGCAAAGCTAT
CACTTGGATGTCGACCCCGATTAAAGGCCTGGGCCACCGTAGTCCAGATTCCTTGCTGGAGACCGAAACCGGTGCATTGG
AAGTCTGTGATTTGATTGGACGCCTGGAGCACGGCGTATTTTCATAA
ATGAGAGCGTATCACCCCACTCCAGCGACCAAAACAAGGGCGCTATGGCGAGAGATTGGTTTGCCCGCCAGCCGCGGTAC
GGTATTGGTCGATAGCATCAAAATGGGTTTTTCTGTTGATGTCATTGACAGCATCCATCTATGGGCATCTATCCCAAAAG
CTGAAATCTTGCGTGCGACCGGTATTCCCAGCCGCAGCTTGACTCGCAGACGCACCCATGATGGCCGTTTCACTCCCGAA
GAGAGTGAGCGCATTGCGCGTTTCGTTCGGGTTATGGACGCCGCAGTTGATCTATTTGGCGGAGATAAAGGCAAAGCTAT
CACTTGGATGTCGACCCCGATTAAAGGCCTGGGCCACCGTAGTCCAGATTCCTTGCTGGAGACCGAAACCGGTGCATTGG
AAGTCTGTGATTTGATTGGACGCCTGGAGCACGGCGTATTTTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1Q1V8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 2ICT | |
| PDB | 2ICP | |
| AlphaFold DB | A0A829CUG6 |