Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1898179..1898681 Replicon chromosome
Accession NZ_CP092532
Organism Escherichia coli strain DS566-2

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag MKD45_RS08970 Protein ID WP_000767822.1
Coordinates 1898427..1898681 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MKD45_RS08965 Protein ID WP_001259255.1
Coordinates 1898179..1898430 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD45_RS08940 (1893357) 1893357..1894424 - 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
MKD45_RS08945 (1894424) 1894424..1895494 - 1071 WP_001538918.1 histidinol-phosphate transaminase -
MKD45_RS08950 (1895491) 1895491..1896795 - 1305 WP_001445483.1 histidinol dehydrogenase -
MKD45_RS08955 (1896801) 1896801..1897700 - 900 WP_000131779.1 ATP phosphoribosyltransferase -
MKD45_RS08960 (1897846) 1897846..1897896 - 51 WP_001364200.1 his operon leader peptide -
MKD45_RS08965 (1898179) 1898179..1898430 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MKD45_RS08970 (1898427) 1898427..1898681 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MKD45_RS08975 (1898764) 1898764..1899588 + 825 WP_000754737.1 SDR family oxidoreductase -
MKD45_RS08980 (1899634) 1899634..1900563 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
MKD45_RS08985 (1900778) 1900778..1900840 + 63 WP_010723108.1 membrane protein YoeI -
MKD45_RS08990 (1900830) 1900830..1902188 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T236373 WP_000767822.1 NZ_CP092532:1898427-1898681 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T236373 NZ_LR588412:c4649078-4648794 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT236373 WP_001259255.1 NZ_CP092532:1898179-1898430 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT236373 NZ_LR588412:c4649310-4649068 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References