Detailed information of TA system TA236370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 726259..726952
• Replicon: chromosome
• Accession: NZ_CP092532
• Organism: Escherichia coli strain DS566-2

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: MKD45_RS03520
• Protein ID: WP_000415584.1
• Coordinates: 726259..726555 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: MKD45_RS03525
• Protein ID: WP_000650107.1
• Coordinates: 726557..726952 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD45_RS03485 (721347)	721347..721661	-	315	WP_000958598.1	putative quinol monooxygenase	-
MKD45_RS03490 (721692)	721692..722273	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
MKD45_RS03495 (722592)	722592..722924	+	333	WP_000917685.1	DUF2645 family protein	-
MKD45_RS03500 (722970)	722970..724319	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
MKD45_RS03505 (724316)	724316..724975	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
MKD45_RS03510 (725127)	725127..725519	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MKD45_RS03515 (725572)	725572..726054	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
MKD45_RS03520 (726259)	726259..726555	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
MKD45_RS03525 (726557)	726557..726952	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
MKD45_RS03530 (727085)	727085..728692	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MKD45_RS03535 (728830)	728830..731088	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T236370 WP_000415584.1 NZ_CP092532:726259-726555 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T236370 NZ_LR588412:c3741799-3741482 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCGATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT236370 WP_000650107.1 NZ_CP092532:726557-726952 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT236370 NZ_LR588412:c3741489-3741202 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65