Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 183564..184288 | Replicon | plasmid p1 |
| Accession | NZ_CP092513 | ||
| Organism | Klebsiella quasipneumoniae strain EF262 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A6B7Q840 |
| Locus tag | MKM89_RS26685 | Protein ID | WP_065905253.1 |
| Coordinates | 183977..184288 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | MKM89_RS26680 | Protein ID | WP_049010942.1 |
| Coordinates | 183564..183980 (-) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKM89_RS26650 (MKM89_26645) | 179157..179564 | + | 408 | WP_224396658.1 | transglycosylase SLT domain-containing protein | - |
| MKM89_RS26655 (MKM89_26650) | 179602..180132 | - | 531 | WP_082226180.1 | antirestriction protein | - |
| MKM89_RS26660 (MKM89_26655) | 180757..181590 | - | 834 | WP_065905247.1 | N-6 DNA methylase | - |
| MKM89_RS26665 (MKM89_26660) | 181637..181867 | - | 231 | WP_065905248.1 | hypothetical protein | - |
| MKM89_RS26670 (MKM89_26665) | 181956..182303 | - | 348 | WP_023292162.1 | hypothetical protein | - |
| MKM89_RS26675 (MKM89_26670) | 182369..182749 | - | 381 | WP_065905249.1 | hypothetical protein | - |
| MKM89_RS26680 (MKM89_26675) | 183564..183980 | - | 417 | WP_049010942.1 | helix-turn-helix domain-containing protein | Antitoxin |
| MKM89_RS26685 (MKM89_26680) | 183977..184288 | - | 312 | WP_065905253.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| MKM89_RS26690 (MKM89_26685) | 184395..184619 | - | 225 | WP_049157091.1 | hypothetical protein | - |
| MKM89_RS26695 (MKM89_26690) | 184630..184842 | - | 213 | WP_065905255.1 | hypothetical protein | - |
| MKM89_RS26700 (MKM89_26695) | 184903..185232 | - | 330 | WP_074439645.1 | hypothetical protein | - |
| MKM89_RS26705 (MKM89_26700) | 185229..185465 | - | 237 | WP_227524536.1 | single-stranded DNA-binding protein | - |
| MKM89_RS26710 (MKM89_26705) | 186017..186079 | - | 63 | Protein_202 | hypothetical protein | - |
| MKM89_RS26715 (MKM89_26710) | 186094..186444 | - | 351 | WP_047722605.1 | hypothetical protein | - |
| MKM89_RS26720 (MKM89_26715) | 186441..186713 | - | 273 | WP_065905257.1 | hypothetical protein | - |
| MKM89_RS26725 (MKM89_26720) | 186903..187388 | + | 486 | WP_065905260.1 | cytoplasmic protein | - |
| MKM89_RS26730 (MKM89_26725) | 187634..187753 | - | 120 | WP_223156217.1 | type I toxin-antitoxin system Hok family toxin | - |
| MKM89_RS26735 (MKM89_26730) | 187698..187850 | - | 153 | WP_224230667.1 | DUF5431 family protein | - |
| MKM89_RS26740 (MKM89_26735) | 188009..188335 | - | 327 | WP_038992719.1 | hypothetical protein | - |
| MKM89_RS26745 (MKM89_26740) | 188332..189060 | - | 729 | WP_040107916.1 | plasmid SOS inhibition protein A | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..203351 | 203351 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12525.37 Da Isoelectric Point: 9.9242
>T236367 WP_065905253.1 NZ_CP092513:c184288-183977 [Klebsiella quasipneumoniae]
VHVISRAPFDEAARHYPNDAVAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE
VHVISRAPFDEAARHYPNDAVAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE
Download Length: 312 bp
>T236367 NZ_LR588412:803924-804334 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
Antitoxin
Download Length: 139 a.a. Molecular weight: 15438.52 Da Isoelectric Point: 4.4711
>AT236367 WP_049010942.1 NZ_CP092513:c183980-183564 [Klebsiella quasipneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD
Download Length: 417 bp
>AT236367 NZ_LR588412:803677-803943 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|