Detailed information of TA system TA236349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4629452..4630054
• Replicon: chromosome
• Accession: NZ_CP092510
• Organism: Salmonella enterica subsp. enterica serovar Thompson

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: MKE90_RS22550
• Protein ID: WP_001159635.1
• Coordinates: 4629743..4630054 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKE90_RS22545
• Protein ID: WP_000362050.1
• Coordinates: 4629452..4629742 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKE90_RS22530 (4626945)	4626945..4627847	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
MKE90_RS22535 (4627844)	4627844..4628479	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
MKE90_RS22540 (4628476)	4628476..4629405	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MKE90_RS22545 (4629452)	4629452..4629742	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
MKE90_RS22550 (4629743)	4629743..4630054	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
MKE90_RS22555 (4630272)	4630272..4631201	+	930	WP_001127708.1	alpha/beta hydrolase	-
MKE90_RS22560 (4631287)	4631287..4631598	+	312	WP_017465947.1	type II toxin-antitoxin system HigB family toxin	-
MKE90_RS22565 (4631595)	4631595..4632041	+	447	WP_017465948.1	type II toxin-antitoxin system HigA family antitoxin	-
MKE90_RS22570 (4632056)	4632056..4632997	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MKE90_RS22575 (4633042)	4633042..4633479	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
MKE90_RS22580 (4633476)	4633476..4634348	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MKE90_RS22585 (4634342)	4634342..4634941	-	600	WP_001621364.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T236349 WP_001159635.1 NZ_CP092510:c4630054-4629743 [Salmonella enterica subsp. enterica serovar Thompson]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T236349 NZ_LR588408:339660-340028 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT236349 WP_000362050.1 NZ_CP092510:c4629742-4629452 [Salmonella enterica subsp. enterica serovar Thompson]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT236349 NZ_LR588408:339442-339663 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97