Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HTH(antitoxin) |
| Location | 4264271..4264873 | Replicon | chromosome |
| Accession | NZ_CP092500 | ||
| Organism | Escherichia coli strain A30 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U9XIS6 |
| Locus tag | LU695_RS21190 | Protein ID | WP_000897305.1 |
| Coordinates | 4264562..4264873 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LU695_RS21185 | Protein ID | WP_000356397.1 |
| Coordinates | 4264271..4264561 (-) | Length | 97 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LU695_RS21155 (4259420) | 4259420..4260322 | + | 903 | WP_000331377.1 | formate dehydrogenase O subunit beta | - |
| LU695_RS21160 (4260319) | 4260319..4260954 | + | 636 | WP_000829013.1 | formate dehydrogenase cytochrome b556 subunit | - |
| LU695_RS21165 (4260951) | 4260951..4261880 | + | 930 | WP_000027708.1 | formate dehydrogenase accessory protein FdhE | - |
| LU695_RS21170 (4262210) | 4262210..4262452 | - | 243 | WP_001086388.1 | protein YiiF | - |
| LU695_RS21175 (4262520) | 4262520..4263217 | + | 698 | WP_103215986.1 | IS1-like element IS1A family transposase | - |
| LU695_RS21180 (4263448) | 4263448..4263666 | - | 219 | WP_001295676.1 | CopG family transcriptional regulator | - |
| LU695_RS21185 (4264271) | 4264271..4264561 | - | 291 | WP_000356397.1 | NadS family protein | Antitoxin |
| LU695_RS21190 (4264562) | 4264562..4264873 | - | 312 | WP_000897305.1 | hypothetical protein | Toxin |
| LU695_RS21195 (4265102) | 4265102..4266010 | + | 909 | WP_001162704.1 | alpha/beta hydrolase | - |
| LU695_RS21200 (4266074) | 4266074..4267015 | - | 942 | WP_001297068.1 | fatty acid biosynthesis protein FabY | - |
| LU695_RS21205 (4267060) | 4267060..4267497 | - | 438 | WP_000560983.1 | D-aminoacyl-tRNA deacylase | - |
| LU695_RS21210 (4267494) | 4267494..4268366 | - | 873 | WP_000920762.1 | virulence factor BrkB family protein | - |
| LU695_RS21215 (4268360) | 4268360..4268959 | - | 600 | WP_001315111.1 | glucose-1-phosphatase | - |
| LU695_RS21220 (4269058) | 4269058..4269675 | - | 618 | Protein_4153 | DeoR/GlpR family DNA-binding transcription regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 4262714..4263217 | 503 | |
| - | flank | IS/Tn | - | - | 4269687..4270190 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12190.19 Da Isoelectric Point: 9.7791
>T236328 WP_000897305.1 NZ_CP092500:c4264873-4264562 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
Download Length: 312 bp
>T236328 NZ_LR536431:730918-731232 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 97 a.a. Molecular weight: 10846.43 Da Isoelectric Point: 10.8907
>AT236328 WP_000356397.1 NZ_CP092500:c4264561-4264271 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
Download Length: 291 bp
>AT236328 NZ_LR536431:731229-731645 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|