Detailed information of TA system TA236311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 991074..991801
• Replicon: chromosome
• Accession: NZ_CP092500
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LU695_RS04885
• Protein ID: WP_000547564.1
• Coordinates: 991074..991385 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LU695_RS04890
• Protein ID: WP_000126294.1
• Coordinates: 991382..991801 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU695_RS04855 (986224)	986224..987933	+	1710	WP_097496240.1	formate hydrogenlyase subunit HycE	-
LU695_RS04860 (987943)	987943..988485	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LU695_RS04865 (988485)	988485..989252	+	768	WP_000067392.1	formate hydrogenlyase subunit HycG	-
LU695_RS04870 (989249)	989249..989659	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LU695_RS04875 (989652)	989652..990122	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LU695_RS04880 (990165)	990165..990900	+	736	Protein_960	hypothetical protein	-
LU695_RS04885 (991074)	991074..991385	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LU695_RS04890 (991382)	991382..991801	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LU695_RS04895 (991916)	991916..993340	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
LU695_RS04900 (993349)	993349..994806	-	1458	WP_001107862.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LU695_RS04905 (995066)	995066..996076	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
LU695_RS04910 (996225)	996225..996752	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T236311 WP_000547564.1 NZ_CP092500:991074-991385 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T236311 NZ_LR536430:c1728835-1728539 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT236311 WP_000126294.1 NZ_CP092500:991382-991801 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT236311 NZ_LR536430:c1728537-1728142 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5