Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 550983..551782 Replicon chromosome
Accession NZ_CP092500
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LU695_RS02690 Protein ID WP_000347273.1
Coordinates 550983..551447 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LU695_RS02695 Protein ID WP_001307405.1
Coordinates 551447..551782 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU695_RS02660 (545984) 545984..546418 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LU695_RS02665 (546436) 546436..547314 - 879 WP_001509042.1 PTS N-acetylgalactosamine transporter subunit IID -
LU695_RS02670 (547304) 547304..548083 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LU695_RS02675 (548094) 548094..548567 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LU695_RS02680 (548590) 548590..549870 - 1281 WP_000681922.1 tagatose-bisphosphate aldolase subunit KbaZ -
LU695_RS02685 (550119) 550119..550928 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
LU695_RS02690 (550983) 550983..551447 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LU695_RS02695 (551447) 551447..551782 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LU695_RS02700 (551931) 551931..553502 - 1572 WP_001273771.1 galactarate dehydratase -
LU695_RS02705 (553877) 553877..555211 + 1335 WP_166431787.1 galactarate/glucarate/glycerate transporter GarP -
LU695_RS02710 (555227) 555227..555997 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T236307 WP_000347273.1 NZ_CP092500:c551447-550983 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T236307 NZ_LR536430:c825471-825368 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236307 WP_001307405.1 NZ_CP092500:c551782-551447 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236307 NZ_LR536430:825330-825601 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References