236304

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1981733..1982297 Replicon chromosome
Accession NZ_CP092498
Organism Lacticaseibacillus paracasei strain VHProbi OF10

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag MKL87_RS09325 Protein ID WP_016363448.1
Coordinates 1981733..1982035 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag MKL87_RS09330 Protein ID WP_003570051.1
Coordinates 1982028..1982297 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKL87_RS09305 (MKL87_09280) 1977046..1977603 + 558 WP_003584278.1 helix-turn-helix transcriptional regulator -
MKL87_RS09310 (MKL87_09285) 1978028..1980205 - 2178 WP_003570057.1 Tex family protein -
MKL87_RS09315 (MKL87_09290) 1980441..1981133 - 693 WP_003570055.1 hypothetical protein -
MKL87_RS09320 (MKL87_09295) 1981251..1981394 - 144 WP_162259784.1 hypothetical protein -
MKL87_RS09325 (MKL87_09300) 1981733..1982035 - 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
MKL87_RS09330 (MKL87_09305) 1982028..1982297 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MKL87_RS09335 (MKL87_09310) 1982671..1983324 - 654 WP_003570049.1 N-acetyltransferase -
MKL87_RS09340 (MKL87_09315) 1983369..1983998 - 630 WP_144182028.1 DUF2399 domain-containing protein -
MKL87_RS09345 (MKL87_09320) 1984061..1984387 - 327 WP_003584272.1 YrdB family protein -
MKL87_RS09350 (MKL87_09325) 1984380..1984784 - 405 WP_003570045.1 MerR family transcriptional regulator -
MKL87_RS09355 (MKL87_09330) 1984982..1986208 + 1227 WP_003570043.1 hypothetical protein -
MKL87_RS09360 (MKL87_09335) 1986405..1987292 + 888 WP_144182030.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T236304 WP_016363448.1 NZ_CP092498:c1982035-1981733 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T236304 NZ_LR536430:c250739-250545 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT236304 WP_003570051.1 NZ_CP092498:c1982297-1982028 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT236304 NZ_LR536430:250368-250546 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References