Detailed information of TA system TA236297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4646648..4647318
• Replicon: chromosome
• Accession: NZ_CP092493
• Organism: Citrobacter freundii strain SCLZS47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MJ253_RS23010
• Protein ID: WP_134216415.1
• Coordinates: 4647016..4647318 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ253_RS23005
• Protein ID: WP_046671375.1
• Coordinates: 4646648..4646989 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ253_RS22975 (4642189)	4642189..4642947	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
MJ253_RS22980 (4643037)	4643037..4643717	+	681	WP_134216417.1	phosphonate C-P lyase system protein PhnL	-
MJ253_RS22985 (4643714)	4643714..4644850	+	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
MJ253_RS22990 (4644853)	4644853..4645407	+	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
MJ253_RS22995 (4645394)	4645394..4645828	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
MJ253_RS23000 (4645837)	4645837..4646595	+	759	WP_134216416.1	phosphonate metabolism protein PhnP	-
MJ253_RS23005 (4646648)	4646648..4646989	-	342	WP_046671375.1	HigA family addiction module antitoxin	Antitoxin
MJ253_RS23010 (4647016)	4647016..4647318	-	303	WP_134216415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ253_RS23015 (4647474)	4647474..4647803	-	330	WP_003031807.1	DDRRRQL repeat protein YjdP	-
MJ253_RS23020 (4647873)	4647873..4650137	-	2265	WP_134216414.1	hybrid sensor histidine kinase/response regulator	-
MJ253_RS23025 (4650252)	4650252..4651775	+	1524	WP_003031804.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11835.48 Da
• Isoelectric Point: 9.9581

>T236297 WP_134216415.1 NZ_CP092493:c4647318-4647016 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKILADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T236297 NZ_LR217818:c4297224-4296937 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT236297 WP_046671375.1 NZ_CP092493:c4646989-4646648 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT236297 NZ_LR217818:c4296950-4296648 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA