Detailed information of TA system TA236243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 344180..344697
• Replicon: chromosome
• Accession: NZ_CP092475
• Organism: Staphylococcus aureus strain P5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: MKD48_RS01645
• Protein ID: WP_000113261.1
• Coordinates: 344431..344697 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: MKD48_RS01640
• Protein ID: WP_000584499.1
• Coordinates: 344180..344431 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKD48_RS01610 (339892)	339892..340611	+	720	WP_000479573.1	amino acid ABC transporter permease	-
MKD48_RS01615 (340608)	340608..341339	+	732	WP_000615455.1	amino acid ABC transporter ATP-binding protein	-
MKD48_RS01620 (341424)	341424..342674	-	1251	WP_001012231.1	FemA/FemB family glycyltransferase FmhA	-
MKD48_RS01625 (342929)	342929..343291	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
MKD48_RS01630 (343310)	343310..343909	+	600	WP_000162810.1	DsbA family protein	-
MKD48_RS01635 (343916)	343916..344017	+	102	Protein_326	hypothetical protein	-
MKD48_RS01640 (344180)	344180..344431	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKD48_RS01645 (344431)	344431..344697	+	267	WP_000113261.1	Txe/YoeB family addiction module toxin	Toxin
MKD48_RS01650 (344732)	344732..344803	+	72	WP_223200558.1	hypothetical protein	-
MKD48_RS01655 (344991)	344991..346538	+	1548	WP_053000508.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
MKD48_RS01660 (346724)	346724..347191	+	468	WP_000153345.1	SRPBCC domain-containing protein	-
MKD48_RS01665 (347278)	347278..347460	+	183	WP_000250735.1	hypothetical protein	-
MKD48_RS01670 (347663)	347663..348487	+	825	WP_000594637.1	formate/nitrite transporter family protein	-
MKD48_RS01675 (348721)	348721..349257	+	537	WP_000169319.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10452.02 Da
• Isoelectric Point: 10.0373

>T236243 WP_000113261.1 NZ_CP092475:344431-344697 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDKHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T236243 NZ_LR213460:2269-2544 [Shigella sonnei]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT236243 WP_000584499.1 NZ_CP092475:344180-344431 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT236243 NZ_LR213460:1985-2269 [Shigella sonnei]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTCATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60