Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2238235..2239154 Replicon chromosome
Accession NC_012207
Organism Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag JTY_RS21180 Protein ID WP_003900449.1
Coordinates 2238549..2239154 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag JTY_RS10455 Protein ID WP_003410124.1
Coordinates 2238235..2238540 (-) Length 102 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTY_RS10425 2233246..2234502 - 1257 WP_003410095.1 HNH endonuclease -
JTY_RS10430 2234856..2235431 + 576 WP_003410100.1 hypothetical protein -
JTY_RS10435 2235428..2236468 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
JTY_RS10440 2236710..2237429 + 720 WP_010950643.1 DUF433 domain-containing protein -
JTY_RS10445 2237419..2237835 + 417 WP_003410114.1 hypothetical protein -
JTY_RS10450 2237851..2238150 - 300 WP_003410120.1 hypothetical protein -
JTY_RS10455 2238235..2238540 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
JTY_RS21180 2238549..2239154 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JTY_RS10465 2239179..2239538 - 360 WP_003410131.1 hypothetical protein -
JTY_RS10470 2239698..2240375 - 678 WP_003900450.1 hypothetical protein -
JTY_RS10475 2240327..2240563 - 237 WP_003410133.1 hypothetical protein -
JTY_RS10480 2240770..2241666 - 897 WP_011799238.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(86-181)

Antitoxin

(32-92)


Sequences


Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T23624 WP_003900449.1 NC_012207:c2239154-2238549 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T23624 NC_012207:c2239154-2238549 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT23624 WP_003410124.1 NC_012207:c2238540-2238235 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT23624 NC_012207:c2238540-2238235 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20


Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References