Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3591102..3591643 Replicon chromosome
Accession NZ_CP092452
Organism Escherichia coli isolate TKEC21-59

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag MJJ11_RS17540 Protein ID WP_000615979.1
Coordinates 3591365..3591643 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MJJ11_RS17535 Protein ID WP_000729704.1
Coordinates 3591102..3591362 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ11_RS17515 (3586785) 3586785..3587570 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
MJJ11_RS17520 (3587542) 3587542..3589254 + 1713 Protein_3448 flagellar biosynthesis protein FlhA -
MJJ11_RS17525 (3589470) 3589470..3589967 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
MJJ11_RS17530 (3590143) 3590143..3590916 - 774 WP_001353765.1 C40 family peptidase -
MJJ11_RS17535 (3591102) 3591102..3591362 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MJJ11_RS17540 (3591365) 3591365..3591643 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MJJ11_RS17545 (3591799) 3591799..3592539 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MJJ11_RS17550 (3592510) 3592510..3593277 - 768 WP_000333379.1 class II glutamine amidotransferase -
MJJ11_RS17555 (3593483) 3593483..3594061 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssA / tssA / hcp1/tssD1 3584564..3614191 29627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T236168 WP_000615979.1 NZ_CP092452:3591365-3591643 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T236168 NZ_LR135444:16991-17143 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATTTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT236168 WP_000729704.1 NZ_CP092452:3591102-3591362 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT236168 NZ_LR135444:17177-17608 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References