Detailed information of TA system TA236167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3584913..3585607
• Replicon: chromosome
• Accession: NZ_CP092452
• Organism: Escherichia coli isolate TKEC21-59

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: MJJ11_RS17500
• Protein ID: WP_001263493.1
• Coordinates: 3584913..3585311 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: MJJ11_RS17505
• Protein ID: WP_000554757.1
• Coordinates: 3585314..3585607 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3580410)	3580410..3580490	-	81	NuclAT_12	-	-
- (3580410)	3580410..3580490	-	81	NuclAT_12	-	-
- (3580410)	3580410..3580490	-	81	NuclAT_12	-	-
- (3580410)	3580410..3580490	-	81	NuclAT_12	-	-
MJJ11_RS17475 (3581086)	3581086..3581544	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
MJJ11_RS17480 (3581805)	3581805..3583262	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
MJJ11_RS17485 (3583319)	3583319..3583840	-	522	Protein_3441	peptide chain release factor H	-
MJJ11_RS17490 (3583839)	3583839..3584042	-	204	Protein_3442	RtcB family protein	-
MJJ11_RS17500 (3584913)	3584913..3585311	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MJJ11_RS17505 (3585314)	3585314..3585607	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MJJ11_RS17510 (3585659)	3585659..3586714	-	1056	WP_001226164.1	DNA polymerase IV	-
MJJ11_RS17515 (3586785)	3586785..3587570	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
MJJ11_RS17520 (3587542)	3587542..3589254	+	1713	Protein_3448	flagellar biosynthesis protein FlhA	-
MJJ11_RS17525 (3589470)	3589470..3589967	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssA / tssA / hcp1/tssD1	3584564..3614191	29627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T236167 WP_001263493.1 NZ_CP092452:c3585311-3584913 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T236167 NZ_LR135443:2608243-2608584 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT236167 WP_000554757.1 NZ_CP092452:c3585607-3585314 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT236167 NZ_LR135443:2608013-2608243 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6