Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 614616..615415 Replicon chromosome
Accession NZ_CP092452
Organism Escherichia coli isolate TKEC21-59

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MJJ11_RS03060 Protein ID WP_000347251.1
Coordinates 614616..615080 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJJ11_RS03065 Protein ID WP_001307405.1
Coordinates 615080..615415 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ11_RS03030 (609616) 609616..610050 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MJJ11_RS03035 (610068) 610068..610946 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MJJ11_RS03040 (610936) 610936..611715 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MJJ11_RS03045 (611726) 611726..612199 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJJ11_RS03050 (612222) 612222..613503 - 1282 Protein_600 tagatose-bisphosphate aldolase subunit KbaZ -
MJJ11_RS03055 (613752) 613752..614561 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJJ11_RS03060 (614616) 614616..615080 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJJ11_RS03065 (615080) 615080..615415 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJJ11_RS03070 (615564) 615564..617135 - 1572 WP_001273753.1 galactarate dehydratase -
MJJ11_RS03075 (617510) 617510..618844 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MJJ11_RS03080 (618860) 618860..619630 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T236149 WP_000347251.1 NZ_CP092452:c615080-614616 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T236149 NZ_LR135408:485750-486091 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236149 WP_001307405.1 NZ_CP092452:c615415-615080 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236149 NZ_LR135408:485520-485750 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References