Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3591878..3592419 Replicon chromosome
Accession NZ_CP092449
Organism Escherichia coli isolate TKEC21-17

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag MJJ13_RS17545 Protein ID WP_000615979.1
Coordinates 3592141..3592419 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MJJ13_RS17540 Protein ID WP_000729704.1
Coordinates 3591878..3592138 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ13_RS17520 (3587561) 3587561..3588346 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
MJJ13_RS17525 (3588318) 3588318..3590030 + 1713 Protein_3450 flagellar biosynthesis protein FlhA -
MJJ13_RS17530 (3590246) 3590246..3590743 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
MJJ13_RS17535 (3590919) 3590919..3591692 - 774 WP_001353765.1 C40 family peptidase -
MJJ13_RS17540 (3591878) 3591878..3592138 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MJJ13_RS17545 (3592141) 3592141..3592419 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MJJ13_RS17550 (3592575) 3592575..3593315 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MJJ13_RS17555 (3593286) 3593286..3594053 - 768 WP_000333379.1 class II glutamine amidotransferase -
MJJ13_RS17560 (3594259) 3594259..3594837 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssA / tssA / hcp1/tssD1 3585340..3614967 29627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T236145 WP_000615979.1 NZ_CP092449:3592141-3592419 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T236145 NZ_LR135401:485750-486091 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT236145 WP_000729704.1 NZ_CP092449:3591878-3592138 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT236145 NZ_LR135401:485520-485750 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References