Detailed information of TA system TA236144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3585689..3586383
• Replicon: chromosome
• Accession: NZ_CP092449
• Organism: Escherichia coli isolate TKEC21-17

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: MJJ13_RS17505
• Protein ID: WP_001263493.1
• Coordinates: 3585689..3586087 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: MJJ13_RS17510
• Protein ID: WP_000554757.1
• Coordinates: 3586090..3586383 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3581186)	3581186..3581266	-	81	NuclAT_12	-	-
- (3581186)	3581186..3581266	-	81	NuclAT_12	-	-
- (3581186)	3581186..3581266	-	81	NuclAT_12	-	-
- (3581186)	3581186..3581266	-	81	NuclAT_12	-	-
MJJ13_RS17480 (3581862)	3581862..3582320	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
MJJ13_RS17485 (3582581)	3582581..3584038	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
MJJ13_RS17490 (3584095)	3584095..3584616	-	522	Protein_3443	peptide chain release factor H	-
MJJ13_RS17495 (3584615)	3584615..3584818	-	204	Protein_3444	RtcB family protein	-
MJJ13_RS17505 (3585689)	3585689..3586087	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MJJ13_RS17510 (3586090)	3586090..3586383	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MJJ13_RS17515 (3586435)	3586435..3587490	-	1056	WP_001226164.1	DNA polymerase IV	-
MJJ13_RS17520 (3587561)	3587561..3588346	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
MJJ13_RS17525 (3588318)	3588318..3590030	+	1713	Protein_3450	flagellar biosynthesis protein FlhA	-
MJJ13_RS17530 (3590246)	3590246..3590743	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / tssA / tssA / hcp1/tssD1	3585340..3614967	29627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T236144 WP_001263493.1 NZ_CP092449:c3586087-3585689 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T236144 NZ_LR135401:198213-198479 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT236144 WP_000554757.1 NZ_CP092449:c3586383-3586090 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT236144 NZ_LR135401:197950-198213 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6