Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 615392..616191 Replicon chromosome
Accession NZ_CP092449
Organism Escherichia coli isolate TKEC21-17

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MJJ13_RS03075 Protein ID WP_000347251.1
Coordinates 615392..615856 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJJ13_RS03080 Protein ID WP_001307405.1
Coordinates 615856..616191 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ13_RS03045 (610392) 610392..610826 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MJJ13_RS03050 (610844) 610844..611722 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MJJ13_RS03055 (611712) 611712..612491 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MJJ13_RS03060 (612502) 612502..612975 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJJ13_RS03065 (612998) 612998..614279 - 1282 Protein_603 tagatose-bisphosphate aldolase subunit KbaZ -
MJJ13_RS03070 (614528) 614528..615337 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJJ13_RS03075 (615392) 615392..615856 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJJ13_RS03080 (615856) 615856..616191 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJJ13_RS03085 (616340) 616340..617911 - 1572 WP_001273753.1 galactarate dehydratase -
MJJ13_RS03090 (618286) 618286..619620 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MJJ13_RS03095 (619636) 619636..620406 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T236126 WP_000347251.1 NZ_CP092449:c615856-615392 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T236126 NZ_LR135359:8081-8338 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236126 WP_001307405.1 NZ_CP092449:c616191-615856 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236126 NZ_LR135359:7819-8088 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References