Detailed information of TA system TA236114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4708624..4709378
• Replicon: chromosome
• Accession: NZ_CP092442
• Organism: Citrobacter portucalensis strain Sb-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5B0T5Y1
• Locus tag: I9P40_RS22360
• Protein ID: WP_048215202.1
• Coordinates: 4708624..4708935 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9P40_RS22365
• Protein ID: WP_048215203.1
• Coordinates: 4708932..4709378 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9P40_RS22330 (I9P40_09675)	4704291..4705193	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
I9P40_RS22335 (I9P40_09670)	4705190..4705825	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
I9P40_RS22340 (I9P40_09665)	4705822..4706751	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
I9P40_RS22345 (I9P40_09660)	4706788..4707162	-	375	WP_020996118.1	type II toxin-antitoxin system VapC family toxin	-
I9P40_RS22350 (I9P40_09655)	4707162..4707404	-	243	WP_020996119.1	CopG family transcriptional regulator	-
I9P40_RS22355 (I9P40_09650)	4707610..4708539	+	930	WP_198802932.1	alpha/beta hydrolase	-
I9P40_RS22360 (I9P40_09645)	4708624..4708935	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	Toxin
I9P40_RS22365 (I9P40_09640)	4708932..4709378	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	Antitoxin
I9P40_RS22370 (I9P40_09635)	4709396..4710337	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
I9P40_RS22375 (I9P40_09630)	4710382..4710819	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
I9P40_RS22380 (I9P40_09625)	4710816..4711688	-	873	WP_008786998.1	virulence factor BrkB family protein	-
I9P40_RS22385 (I9P40_09620)	4711682..4712281	-	600	WP_103285939.1	glucose-1-phosphatase	-
I9P40_RS22390 (I9P40_09615)	4712372..4713262	-	891	WP_103285940.1	aldose epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12345.28 Da
• Isoelectric Point: 9.5337

>T236114 WP_048215202.1 NZ_CP092442:4708624-4708935 [Citrobacter portucalensis]
VHVISRKPFNEAMLMYPNHQLALADLLNVLEKKTFIQPEEMKQYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T236114 NZ_LR135344:200491-200757 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16660.97 Da
• Isoelectric Point: 6.7580

>AT236114 WP_048215203.1 NZ_CP092442:4708932-4709378 [Citrobacter portucalensis]
MRTHRHMDAASAKKIVDTFSDAVKTIPLLGEDRNDTEYRRALALVEYLVDHDDLENPLFELLCARISKYEKHAPEFKALN
QHLEKTPSGVSVLRTLMDQYSLKAADLANELGSKSNVSNILNGRRALTVSHIKALAERFNLPADAFIE

Nucleotide

• Length: 447 bp

>AT236114 NZ_LR135344:200228-200491 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A5B0T5Y1