Detailed information of TA system TA236108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2432771..2433333
• Replicon: chromosome
• Accession: NZ_CP092442
• Organism: Citrobacter portucalensis strain Sb-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I9P40_RS11535
• Protein ID: WP_048216313.1
• Coordinates: 2433055..2433333 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: I9P40_RS11530
• Protein ID: WP_003833018.1
• Coordinates: 2432771..2433055 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9P40_RS11515 (I9P40_20485)	2430466..2431350	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
I9P40_RS11520 (I9P40_20480)	2431343..2431999	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
I9P40_RS11525 (I9P40_20475)	2432129..2432731	+	603	WP_191227172.1	inorganic diphosphatase	-
I9P40_RS11530 (I9P40_20470)	2432771..2433055	-	285	WP_003833018.1	HigA family addiction module antitoxin	Antitoxin
I9P40_RS11535 (I9P40_20465)	2433055..2433333	-	279	WP_048216313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9P40_RS11540 (I9P40_20460)	2433554..2435269	-	1716	WP_198802214.1	ABC-F family ATP-binding cassette domain-containing protein	-
I9P40_RS11545 (I9P40_20455)	2435579..2437276	-	1698	WP_003833011.1	malate dehydrogenase	-
I9P40_RS11550 (I9P40_20450)	2437456..2437596	-	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
I9P40_RS11555 (I9P40_20445)	2437824..2438255	+	432	WP_005126807.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10681.33 Da
• Isoelectric Point: 9.8965

>T236108 WP_048216313.1 NZ_CP092442:c2433333-2433055 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTEINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T236108 NZ_LR135331:1254084-1254425 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT236108 WP_003833018.1 NZ_CP092442:c2433055-2432771 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT236108 NZ_LR135331:1253854-1254084 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8