Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 328666..329332 Replicon chromosome
Accession NZ_CP092442
Organism Citrobacter portucalensis strain Sb-2

Toxin (Protein)


Gene name higB Uniprot ID A0A5B0T6K1
Locus tag I9P40_RS01515 Protein ID WP_047408175.1
Coordinates 329015..329332 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5B0T6F5
Locus tag I9P40_RS01510 Protein ID WP_048236945.1
Coordinates 328666..328962 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9P40_RS01495 (I9P40_07300) 325111..325584 - 474 WP_198802897.1 transcription elongation factor GreB -
I9P40_RS01500 (I9P40_07295) 325810..326529 + 720 WP_001157751.1 two-component system response regulator OmpR -
I9P40_RS01505 (I9P40_07290) 326526..327878 + 1353 WP_003827658.1 two-component system sensor histidine kinase EnvZ -
I9P40_RS01510 (I9P40_07285) 328666..328962 - 297 WP_048236945.1 NadS family protein Antitoxin
I9P40_RS01515 (I9P40_07280) 329015..329332 - 318 WP_047408175.1 toxin HigB-2 Toxin
I9P40_RS01520 (I9P40_07275) 329455..331077 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
I9P40_RS01525 (I9P40_07270) 331456..333174 + 1719 WP_198802899.1 DUF4153 domain-containing protein -
I9P40_RS01530 (I9P40_07265) 333284..334162 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12255.17 Da        Isoelectric Point: 10.0909

>T236097 WP_047408175.1 NZ_CP092442:c329332-329015 [Citrobacter portucalensis]
MFTFIELQGFSKRRPLLLPDDEFRAFQETLIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T236097 NZ_LR135317:190006-190272 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11185.95 Da        Isoelectric Point: 7.2052

>AT236097 WP_048236945.1 NZ_CP092442:c328962-328666 [Citrobacter portucalensis]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQRNPFIINQLKAI

Download         Length: 297 bp

>AT236097 NZ_LR135317:189743-190006 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5B0T6K1

Antitoxin

Source ID Structure
AlphaFold DB A0A5B0T6F5

References