Detailed information of TA system TA236066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4169118..4169854
• Replicon: chromosome
• Accession: NZ_CP092417
• Organism: Endozoicomonas sp. SCSIO W0465

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MJO57_RS18840
• Protein ID: WP_252017881.1
• Coordinates: 4169118..4169432 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJO57_RS18845
• Protein ID: WP_252017883.1
• Coordinates: 4169429..4169854 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJO57_RS18815 (MJO57_18740)	4164350..4164730	-	381	WP_252017874.1	hypothetical protein	-
MJO57_RS18820 (MJO57_18745)	4165265..4166530	-	1266	WP_252017875.1	group II intron reverse transcriptase/maturase	-
MJO57_RS18825 (MJO57_18750)	4167109..4167396	-	288	WP_252017876.1	hypothetical protein	-
MJO57_RS18830 (MJO57_18755)	4167509..4168615	+	1107	WP_252017878.1	hypothetical protein	-
MJO57_RS18835 (MJO57_18760)	4168778..4168948	+	171	WP_252017880.1	hypothetical protein	-
MJO57_RS18840 (MJO57_18765)	4169118..4169432	+	315	WP_252017881.1	type II toxin-antitoxin system HigB family toxin	Toxin
MJO57_RS18845 (MJO57_18770)	4169429..4169854	+	426	WP_252017883.1	helix-turn-helix domain-containing protein	Antitoxin
MJO57_RS18850 (MJO57_18775)	4169950..4170162	-	213	WP_252017885.1	hypothetical protein	-
MJO57_RS18855 (MJO57_18780)	4170943..4172499	+	1557	WP_252017887.1	IS21 family transposase	-
MJO57_RS18860 (MJO57_18785)	4172512..4173267	+	756	WP_252017309.1	IS21-like element helper ATPase IstB	-
MJO57_RS18865 (MJO57_18790)	4173271..4174326	-	1056	WP_252017889.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4052416..4173267	120851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (8-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12107.79 Da
• Isoelectric Point: 9.7655

>T236066 WP_252017881.1 NZ_CP092417:4169118-4169432 [Endozoicomonas sp. SCSIO W0465]
MHVISRKPFHDAAKKYPNDAAAIDAAYKALRNGDFSSPKELKALFPNLDNFKYKDKWWIIDIGGNNLRLIAFIEFRDNRM
YVKHIVSHSEYDKLSKNYAKEADS

Nucleotide

• Length: 315 bp

>T236066 NZ_LR135254:472121-472462 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 16459.67 Da
• Isoelectric Point: 4.6708

>AT236066 WP_252017883.1 NZ_CP092417:4169429-4169854 [Endozoicomonas sp. SCSIO W0465]
MRYANLKEKAVDFFAEARFISRIHNESEYQQALELMDELIEDYDRYLPLIEVLSVAIEKWEDESAECSEFNRRIEALDDG
VAILRTLMDQYHLKADDLKTELGSKSLVSMILNGTRNLTRDHIQALSERFKISPSVFFHKA

Nucleotide

• Length: 426 bp

>AT236066 NZ_LR135254:471891-472121 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG