Detailed information of TA system TA236052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 111885..112612
• Replicon: plasmid Kp116-unnamed1
• Accession: NZ_CP092410
• Organism: Klebsiella pneumoniae strain IA-044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KZ664_RS25725
• Protein ID: WP_011251285.1
• Coordinates: 111885..112196 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ664_RS25730
• Protein ID: WP_011251286.1
• Coordinates: 112193..112612 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ664_RS25695 (KZ664_25855)	107114..108094	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ664_RS25700 (KZ664_25860)	108641..108820	-	180	Protein_111	IS5/IS1182 family transposase	-
KZ664_RS25705 (KZ664_25865)	108860..110398	-	1539	WP_032430751.1	IS66 family transposase	-
KZ664_RS25710 (KZ664_25870)	110447..110794	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
KZ664_RS25715 (KZ664_25875)	110791..111129	-	339	WP_023288266.1	transposase	-
KZ664_RS25720 (KZ664_25880)	111243..111680	+	438	Protein_115	DDE-type integrase/transposase/recombinase	-
KZ664_RS25725 (KZ664_25885)	111885..112196	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZ664_RS25730 (KZ664_25890)	112193..112612	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KZ664_RS25735 (KZ664_25895)	112682..112984	+	303	WP_167813119.1	hypothetical protein	-
KZ664_RS25740 (KZ664_25900)	113032..114012	-	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ664_RS25745 (KZ664_25905)	114014..114559	+	546	Protein_120	methyl-accepting chemotaxis protein	-
KZ664_RS25750 (KZ664_25910)	115135..115341	+	207	Protein_121	hypothetical protein	-
KZ664_RS25755 (KZ664_25915)	115801..116769	+	969	WP_000654811.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheD	1..175354	175354
	-	inside	IScluster/Tn	-	cheD	107114..118998	11884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T236052 WP_011251285.1 NZ_CP092410:111885-112196 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T236052 NZ_LR135235:476831-477172 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT236052 WP_011251286.1 NZ_CP092410:112193-112612 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT236052 NZ_LR135235:476601-476831 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1