Detailed information of TA system TA236049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 20090..20817
• Replicon: plasmid Kp25-unnamed1
• Accession: NZ_CP092408
• Organism: Klebsiella quasipneumoniae strain MN-07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KZ661_RS25295
• Protein ID: WP_011251285.1
• Coordinates: 20090..20401 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ661_RS25300
• Protein ID: WP_011251286.1
• Coordinates: 20398..20817 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ661_RS25265 (KZ661_25455)	15867..16217	-	351	WP_004189161.1	IS66 family insertion sequence element accessory protein TnpB	-
KZ661_RS25270 (KZ661_25460)	16214..16654	-	441	WP_240247758.1	transposase	-
KZ661_RS25275 (KZ661_25465)	16768..17996	+	1229	Protein_17	IS3 family transposase	-
KZ661_RS25280 (KZ661_25470)	18447..19144	+	698	WP_240247761.1	IS1 family transposase	-
KZ661_RS25285 (KZ661_25475)	19155..19322	-	168	WP_232690621.1	transposase	-
KZ661_RS25290 (KZ661_25480)	19448..19885	+	438	Protein_20	DDE-type integrase/transposase/recombinase	-
KZ661_RS25295 (KZ661_25485)	20090..20401	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZ661_RS25300 (KZ661_25490)	20398..20817	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KZ661_RS25305 (KZ661_25495)	20964..21932	+	969	WP_186983401.1	IS5 family transposase	-
KZ661_RS25310 (KZ661_25500)	22338..23705	-	1368	WP_023329010.1	formimidoylglutamate deiminase	-
KZ661_RS25315 (KZ661_25505)	23808..24569	+	762	WP_017900871.1	histidine utilization repressor	-
KZ661_RS25320 (KZ661_25510)	24566..25123	+	558	WP_017900872.1	HutD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..223240	223240
	-	inside	IScluster/Tn	-	-	14295..21932	7637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T236049 WP_011251285.1 NZ_CP092408:20090-20401 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T236049 NZ_LR135228:49036-49386 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT236049 WP_011251286.1 NZ_CP092408:20398-20817 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT236049 NZ_LR135228:48779-49042 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1