Detailed information of TA system TA236046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 128404..129131
• Replicon: plasmid Kp62-unnamed1
• Accession: NZ_CP092407
• Organism: Klebsiella pneumoniae strain CT-04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KZ663_RS26450
• Protein ID: WP_011251285.1
• Coordinates: 128404..128715 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ663_RS26455
• Protein ID: WP_011251286.1
• Coordinates: 128712..129131 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ663_RS26420 (KZ663_26600)	123634..124614	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ663_RS26425 (KZ663_26605)	125160..125339	-	180	Protein_129	IS5/IS1182 family transposase	-
KZ663_RS26430 (KZ663_26610)	125379..126917	-	1539	WP_032430751.1	IS66 family transposase	-
KZ663_RS26435 (KZ663_26615)	126966..127313	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
KZ663_RS26440 (KZ663_26620)	127310..127648	-	339	WP_023288266.1	transposase	-
KZ663_RS26445 (KZ663_26625)	127762..128199	+	438	Protein_133	DDE-type integrase/transposase/recombinase	-
KZ663_RS26450 (KZ663_26630)	128404..128715	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZ663_RS26455 (KZ663_26635)	128712..129131	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KZ663_RS26460 (KZ663_26640)	129201..129503	+	303	WP_167813119.1	hypothetical protein	-
KZ663_RS26465 (KZ663_26645)	129551..130531	-	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ663_RS26470 (KZ663_26650)	130533..131078	+	546	Protein_138	methyl-accepting chemotaxis protein	-
KZ663_RS26475 (KZ663_26655)	131654..131860	+	207	Protein_139	hypothetical protein	-
KZ663_RS26480 (KZ663_26660)	132408..133403	+	996	WP_223290840.1	FUSC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	cheD	1..189738	189738
	-	inside	IScluster/Tn	-	cheD	123634..134452	10818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T236046 WP_011251285.1 NZ_CP092407:128404-128715 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T236046 NZ_LR135223:24212-24562 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGGGTTCTGTTGCAAAGTTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT236046 WP_011251286.1 NZ_CP092407:128712-129131 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT236046 NZ_LR135223:23938-24210 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1