Detailed information of TA system TA236044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 72433..73160
• Replicon: plasmid Kp46-unnamed1
• Accession: NZ_CP092405
• Organism: Klebsiella pneumoniae strain NY-06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KZ657_RS26220
• Protein ID: WP_011251285.1
• Coordinates: 72433..72744 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ657_RS26225
• Protein ID: WP_011251286.1
• Coordinates: 72741..73160 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ657_RS26190	67662..68642	+	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ657_RS26195	69189..69368	-	180	Protein_66	IS5/IS1182 family transposase	-
KZ657_RS26200	69408..70946	-	1539	WP_032430751.1	IS66 family transposase	-
KZ657_RS26205	70995..71342	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
KZ657_RS26210	71339..71677	-	339	WP_023288266.1	transposase	-
KZ657_RS26215	71791..72228	+	438	Protein_70	DDE-type integrase/transposase/recombinase	-
KZ657_RS26220	72433..72744	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZ657_RS26225	72741..73160	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KZ657_RS26230	73230..73532	+	303	WP_167813119.1	hypothetical protein	-
KZ657_RS26235	73580..74560	-	981	WP_048293850.1	IS5-like element ISKpn26 family transposase	-
KZ657_RS26240	74556..75149	+	594	Protein_75	methyl-accepting chemotaxis protein	-
KZ657_RS26245	75683..75889	+	207	Protein_76	hypothetical protein	-
KZ657_RS26250	76437..77432	+	996	WP_223290840.1	FUSC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheD	1..142759	142759
	-	inside	IScluster/Tn	-	cheD	67662..78481	10819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T236044 WP_011251285.1 NZ_CP092405:72433-72744 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T236044 NZ_LR135221:99060-99317 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT236044 WP_011251286.1 NZ_CP092405:72741-73160 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT236044 NZ_LR135221:98798-99067 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1