Detailed information of TA system TA236023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 41718..42307
• Replicon: chromosome
• Accession: NZ_CP092382
• Organism: Xanthomonas oryzae strain PXO339

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: MJJ08_RS00235
• Protein ID: WP_012443754.1
• Coordinates: 42026..42307 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJJ08_RS00230
• Protein ID: WP_011260746.1
• Coordinates: 41718..42008 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ08_RS00200 (MJJ08_00200)	36862..37479	-	618	WP_082325600.1	DUF4123 domain-containing protein	-
MJJ08_RS00205 (MJJ08_00205)	37482..37904	-	423	WP_042465811.1	PAAR domain-containing protein	-
MJJ08_RS00210 (MJJ08_00210)	38032..38358	-	327	WP_228329596.1	hypothetical protein	-
MJJ08_RS00215 (MJJ08_00215)	38613..38945	+	333	WP_011260743.1	hypothetical protein	-
MJJ08_RS00220 (MJJ08_00220)	39105..39292	+	188	Protein_41	hypothetical protein	-
MJJ08_RS00225 (MJJ08_00225)	39395..40789	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
MJJ08_RS00230 (MJJ08_00230)	41718..42008	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
MJJ08_RS00235 (MJJ08_00235)	42026..42307	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ08_RS00240 (MJJ08_00240)	42402..44654	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T236023 WP_012443754.1 NZ_CP092382:c42307-42026 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T236023 NZ_LR135181:504489-504830 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT236023 WP_011260746.1 NZ_CP092382:c42008-41718 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT236023 NZ_LR135181:504259-504489 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5