Detailed information of TA system TA236018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 541549..542138
• Replicon: chromosome
• Accession: NZ_CP092381
• Organism: Xanthomonas oryzae strain HN2011

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: MJJ07_RS02550
• Protein ID: WP_012443754.1
• Coordinates: 541857..542138 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJJ07_RS02545
• Protein ID: WP_011260746.1
• Coordinates: 541549..541839 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ07_RS02515 (MJJ07_02505)	536702..537310	-	609	WP_113195694.1	DUF4123 domain-containing protein	-
MJJ07_RS02520 (MJJ07_02510)	537313..537735	-	423	WP_042465811.1	PAAR domain-containing protein	-
MJJ07_RS02525 (MJJ07_02515)	537863..538189	-	327	WP_228329596.1	hypothetical protein	-
MJJ07_RS02530 (MJJ07_02520)	538444..538776	+	333	WP_011260743.1	hypothetical protein	-
MJJ07_RS02535 (MJJ07_02525)	538936..539123	+	188	Protein_483	hypothetical protein	-
MJJ07_RS02540 (MJJ07_02530)	539226..540620	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
MJJ07_RS02545 (MJJ07_02535)	541549..541839	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
MJJ07_RS02550 (MJJ07_02540)	541857..542138	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ07_RS02555 (MJJ07_02545)	542233..544485	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T236018 WP_012443754.1 NZ_CP092381:c542138-541857 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T236018 NZ_LR135176:8993-9250 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT236018 WP_011260746.1 NZ_CP092381:c541839-541549 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT236018 NZ_LR135176:8731-9000 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5