Detailed information of TA system TA236017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4547102..4547691
• Replicon: chromosome
• Accession: NZ_CP092380
• Organism: Xanthomonas oryzae strain GD1358

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: MJJ09_RS21365
• Protein ID: WP_012443754.1
• Coordinates: 4547410..4547691 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJJ09_RS21360
• Protein ID: WP_011260746.1
• Coordinates: 4547102..4547392 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ09_RS21330 (MJJ09_21285)	4542255..4542863	-	609	WP_011409746.1	DUF4123 domain-containing protein	-
MJJ09_RS21335 (MJJ09_21290)	4542866..4543288	-	423	WP_042465811.1	PAAR domain-containing protein	-
MJJ09_RS21340 (MJJ09_21295)	4543416..4543742	-	327	WP_228329596.1	hypothetical protein	-
MJJ09_RS21345 (MJJ09_21300)	4543997..4544329	+	333	WP_011260743.1	hypothetical protein	-
MJJ09_RS21350 (MJJ09_21305)	4544489..4544676	+	188	Protein_4073	hypothetical protein	-
MJJ09_RS21355 (MJJ09_21310)	4544779..4546173	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
MJJ09_RS21360 (MJJ09_21315)	4547102..4547392	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
MJJ09_RS21365 (MJJ09_21320)	4547410..4547691	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ09_RS21370 (MJJ09_21325)	4547786..4550038	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T236017 WP_012443754.1 NZ_CP092380:c4547691-4547410 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T236017 NZ_LR135175:102539-102880 [Enterococcus faecium]
ATGACTGAAGAAAAAAATTACATTCCTAAAAAGGGAGATATTGTTTGGATTGATTTTGATCCTTCTGCAGGAAAAGAGAT
TCAAAAGCGGCGTCCTGGATTAGTTGTTTCTAGATACGAGTTTAATCGCAAAACAATGTTTGCAGTTATTTGTCCGATTA
CCTCTACTATAAGAAATTTTCCGACACGCTATTCATTACCTACCGAATTAGACACTACTGGACAAATACTTATTTCTCAA
TTGAAGTCGCTCGATTTCAATGAGAGAAAACTTAAAAAAATTGAATCCTTACCTTTACAAGATATGGCGAAAATCGATCA
AATGATTGAATATATATTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT236017 WP_011260746.1 NZ_CP092380:c4547392-4547102 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT236017 NZ_LR135175:102309-102539 [Enterococcus faecium]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGATTACTTTGCCTTCTGATAACGGAAAGAAGCCTTCGGA
AAATAAAGAATATATAGTTATCTATTCTGATGATGGAACAATTACTTTGGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAAGCGGAGTATTACGAAAAAGATGAATGGGAAGATTTGTCTCCTGAAGGAAGAGAGATTTTATAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5