Detailed information of TA system TA236012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4281798..4282387
• Replicon: chromosome
• Accession: NZ_CP092379
• Organism: Xanthomonas oryzae strain GIV

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: MJJ06_RS20320
• Protein ID: WP_012443754.1
• Coordinates: 4281798..4282079 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJJ06_RS20325
• Protein ID: WP_011260746.1
• Coordinates: 4282097..4282387 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ06_RS20315 (MJJ06_20295)	4279451..4281703	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
MJJ06_RS20320 (MJJ06_20300)	4281798..4282079	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ06_RS20325 (MJJ06_20305)	4282097..4282387	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
MJJ06_RS20330 (MJJ06_20310)	4283316..4284710	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
MJJ06_RS20335 (MJJ06_20315)	4284813..4285000	-	188	Protein_3889	hypothetical protein	-
MJJ06_RS20340 (MJJ06_20320)	4285160..4285492	-	333	WP_011260743.1	hypothetical protein	-
MJJ06_RS20345 (MJJ06_20325)	4285747..4286073	+	327	WP_228329596.1	hypothetical protein	-
MJJ06_RS20350 (MJJ06_20330)	4286201..4286623	+	423	WP_042465811.1	PAAR domain-containing protein	-
MJJ06_RS20355 (MJJ06_20335)	4286626..4287234	+	609	WP_011409746.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T236012 WP_012443754.1 NZ_CP092379:4281798-4282079 [Xanthomonas oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T236012 NZ_LR135169:485096-485440 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCATTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACACCTGAATAATTCATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT236012 WP_011260746.1 NZ_CP092379:4282097-4282387 [Xanthomonas oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT236012 NZ_LR135169:484866-485096 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5