Detailed information of TA system TA235933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 34528..35202
• Replicon: plasmid pKPN20-1
• Accession: NZ_CP092337
• Organism: Klebsiella pneumoniae strain KPN20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: MG291_RS25895
• Protein ID: WP_032720638.1
• Coordinates: 34879..35202 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MG291_RS25890
• Protein ID: WP_032720637.1
• Coordinates: 34528..34830 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MG291_RS25850 (MG291_25850)	29721..30293	+	573	WP_044266753.1	hypothetical protein	-
MG291_RS25855 (MG291_25855)	30324..30818	+	495	WP_044266755.1	hypothetical protein	-
MG291_RS25860 (MG291_25860)	31121..32041	+	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
MG291_RS25865 (MG291_25865)	32053..32865	+	813	WP_223203129.1	TSUP family transporter	-
MG291_RS25870 (MG291_25870)	32867..33439	+	573	WP_032720635.1	GNAT family N-acetyltransferase	-
MG291_RS25875 (MG291_25875)	33544..33927	+	384	WP_032720636.1	hypothetical protein	-
MG291_RS25880 (MG291_25880)	33924..34130	+	207	WP_153591439.1	hypothetical protein	-
MG291_RS25885 (MG291_25885)	34318..34485	+	168	WP_153591440.1	hypothetical protein	-
MG291_RS25890 (MG291_25890)	34528..34830	-	303	WP_032720637.1	NadS family protein	Antitoxin
MG291_RS25895 (MG291_25895)	34879..35202	-	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MG291_RS27790	35380..35532	-	153	WP_269233002.1	DUF4113 domain-containing protein	-
MG291_RS27795	35564..35698	+	135	WP_269233001.1	hypothetical protein	-
MG291_RS25905 (MG291_25905)	35763..36049	+	287	Protein_45	DNA-binding protein	-
MG291_RS25910 (MG291_25910)	36104..36781	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
MG291_RS25915 (MG291_25915)	36781..37128	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
MG291_RS25920 (MG291_25920)	37148..38719	+	1572	WP_029392005.1	IS66-like element ISCro1 family transposase	-
MG291_RS25925 (MG291_25925)	38751..38897	+	147	Protein_49	DNA-binding protein	-
MG291_RS25930 (MG291_25930)	38966..39568	-	603	Protein_50	RNA-guided endonuclease TnpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / ARR-3 / rmtF	-	1..212639	212639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T235933 WP_032720638.1 NZ_CP092337:c35202-34879 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T235933 NZ_LR134494:c1159254-1159051 [Serratia quinivorans]
ATGACTAAAATTGATTATCTGATGCGTTTACGTAAATGCACCACGATCGAAACACTGGAACGCGTTATTGAAAAAAACAA
GTATGAGCTTTCAGATGACGAACTGGAGCTGTTTTACTCAGCAGCCGACCATCGCCTGGCCGAGCTCACCATGAATAAAC
TCTACGATAAAATCCCTACTGCCGTTTGGAAATTCGTAAGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT235933 WP_032720637.1 NZ_CP092337:c34830-34528 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT235933 NZ_LR134494:c1159678-1159310 [Serratia quinivorans]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAGCTGAAATTCTTGTGTGAAAACCTGTATGATGAAGGTATCGC
CACGCTGGGCGACAGCCATCATGGCTGGGTGAACGATCCGACGTCTTCCGTCAACCTCCAGCTTAATGAGTTGATCGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAAAGTGATTTATCGGAACTTGTCGAAGAGTACCTC
GATGATACCTACACGCTGTTCAGCAGTTACGGTATAAATGATTCCGATCTGCGTCGTTGGCAGAAAACCAAGGCGCGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5