Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3949704..3950301 Replicon chromosome
Accession NZ_CP092336
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MG291_RS19080 Protein ID WP_004142563.1
Coordinates 3949984..3950301 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MG291_RS19075 Protein ID WP_004142561.1
Coordinates 3949704..3949991 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS19045 (3945784) 3945784..3946032 + 249 WP_020325243.1 DUF1158 domain-containing protein -
MG291_RS19050 (3946050) 3946050..3946391 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
MG291_RS19055 (3946422) 3946422..3947537 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
MG291_RS19060 (3947717) 3947717..3948298 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
MG291_RS19065 (3948298) 3948298..3948666 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
MG291_RS19070 (3948786) 3948786..3949439 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
MG291_RS19075 (3949704) 3949704..3949991 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MG291_RS19080 (3949984) 3949984..3950301 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MG291_RS19085 (3950486) 3950486..3951529 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
MG291_RS19090 (3952199) 3952199..3953065 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
MG291_RS19095 (3953174) 3953174..3954601 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T235926 WP_004142563.1 NZ_CP092336:c3950301-3949984 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T235926 NZ_LR134493:c548508-548140 [Serratia rubidaea]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGCTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAGCAGCACTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTATTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACCGCAGAGGATTT
AGCTCACAGGGACAAGTGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGTGGATTAACGAACAGTTG
TCCTTATCGGGAAGTAAGTAATATTGTTCTTGGCCGATCGCGGCAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT235926 WP_004142561.1 NZ_CP092336:c3949991-3949704 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT235926 NZ_LR134493:c548893-548543 [Serratia rubidaea]
ATGTCGAGTAAGACACTCACAGTGAATGATGATACCGCTGAGCCCTGGTGGGGGCTGAATCGTAATGTCATTCCTTGTTT
TGGTGCGCGTCTGGTCCAGGAAGGTAACCGCCTATATTACCTGTCTGACCGCGCCAGCATAATAGGCCTATTCAATAAAG
CGGATTTGCTCCGTCTTGACCAGGCATTTCCTGTTCTGCTGAAGCAGGCGGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACCCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACGCTGGGTTCCTGTGGTCACGT
CTATATCTCCATTTATCCTACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References