Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 345898..346484 Replicon chromosome
Accession NZ_CP092336
Organism Klebsiella pneumoniae strain KPN20

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MG291_RS01600 Protein ID WP_002920800.1
Coordinates 346116..346484 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MG291_RS01595 Protein ID WP_004174006.1
Coordinates 345898..346119 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG291_RS01575 (342055) 342055..342981 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MG291_RS01580 (342978) 342978..344255 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MG291_RS01585 (344252) 344252..345019 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MG291_RS01590 (345021) 345021..345734 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MG291_RS01595 (345898) 345898..346119 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MG291_RS01600 (346116) 346116..346484 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MG291_RS01605 (346757) 346757..348073 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MG291_RS01610 (348180) 348180..349067 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MG291_RS01615 (349064) 349064..349909 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MG291_RS01620 (349911) 349911..350981 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 342978..351718 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T235914 WP_002920800.1 NZ_CP092336:346116-346484 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T235914 NZ_LR134485:c4690827-4690453 [Citrobacter youngae]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGTGGACGGAGTGAAGCAAAGCAAGCGAT
AGAAACATGGCCGCCGCAGAATGCGATCAGCCTTATCACCTGGATGGAAGTGATGGTTGGGGCAAAGAAATACCATCAGG
AATCCCGCACACGCGTTGCGTTGAGCGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATTATTCTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT235914 WP_004174006.1 NZ_CP092336:345898-346119 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT235914 NZ_LR134485:c4691069-4690827 [Citrobacter youngae]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGAACAGGCTGAAACGA
CTATTTCCAGCGCTCTCGGACTCTGGAAAGGTTGTGAAGAAGATGGCGTTGAGTACCAGCGCAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References